Optimization and application of thermospray high-performance liquid chromatography/mass spectrometry
Optimization and application of thermospray high-performance liquid chromatography/mass spectrometry
复制标题
热喷雾高效液相色谱/质谱联用技术的优化及应用
DOI：
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发表时间：
1985
期刊：
影响因子：
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通讯作者：
C. A. Haney
中科院分区：
文献类型：
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作者：
R. Voyksner;C. A. Haney
Thermospray (TSP) HPLC/MS frequently produces molecular weight information and exhibits lower detection limits than other HPLC/MS techniques for certain compounds. Thermospray exhibits the best sensitivities when used with a 0.1 M ammonium acetate buffer in a high percent water solvent system. The optimal interface temperatures vary with solvent composition. The optimal temperatures can be determined by maximizing the solvent-buffer ion intensities. Thermospray ionization resembles ammonia CI, which produces protonation, ammonium addition, and proton-bound solvent molecule clusters depending on the basicity of the analyte and existing thermodynamics in the source. The ionization process is soft; very few fragment ions are observed. The use of TSP HPLC/MS for the analysis of triazine herbicides and organophosphorus pesticides shows excellent specificity and sensitivity. Thermospray spectra of these compounds were quite simple, consisting of an (M + H)/sup +/ or (M + NH/sub 4/)/sup +/ ion with no fragment ion detected. Detection limits for these compounds varied from 10 to 300 ng and full scan spectra were obtained (S/N of about 5 for the base peak). 36 references, 12 figures, 2 tables.
