Postnatal Development and Functional Adaptations of the Melanopsin Photoreceptive System in the Albino Mouse Retina
Postnatal Development and Functional Adaptations of the Melanopsin Photoreceptive System in the Albino Mouse Retina
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DOI：
10.1167/iovs.10-5253
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发表时间：
2010-09-01
影响因子：
4.4
通讯作者：
Garcia-Fernandez, Jose M.
中科院分区：
文献类型：
--
作者：
Gonzalez-Menendez, Irene;Contreras, Felipe;Garcia-Fernandez, Jose M.
目的。目的研究CD1白化小鼠视网膜黑素系统在出生后发育过程中的变化。方法：将幼鼠置于不同的环境条件下：明暗循环(LD)、恒光(LL)、恒暗(DD)、先明后暗、先明后暗、先暗后暗。应用免疫组织化学方法，根据黑素蛋白表达细胞的胞体和树突的位置，将其分为M1和M2，并进行计数。结果：在LD周期中，免疫反应阳性细胞的数量在出生后第一周内增加。DD组小鼠免疫阳性细胞数的增加明显高于LD组。相反，当小鼠在同一时间内暴露于LL时，没有检测到增加。为了确定LL在出生后早期的影响是否可逆，作者研究了在LL中出生并随后在LD周期下维持的动物。在LD饲养3天后，这些动物的黑素细胞数量显著增加。然而，在LD治疗1个月后，其数量与LD对照组相似。令人惊讶的是，当出生在DD的小鼠暴露在LL中时，没有检测到减少，尽管免疫染色强度很低。结论：每个细胞的黑素蛋白的数量因环境光线条件的不同而不同。黑色素素系统的正常发育可能需要黑暗的周期，或者更可能的是，在日常周期下发生的一系列光明和黑暗的周期。(投资眼科VS科学。2010年；51：48404847)doi：10.1167/iovs.10-5253
PURPOSE. To study the melanopsin system of the albino CD1 mouse retina during postnatal development.METHODS. Pups were kept under different ambient conditions: light/dark (LD) cycles, constant light (LL), constant darkness (DD), LL followed by LD, and DD followed by LL. Using immunohistochemistry, melanopsin-expressing cells were classified as M1 or M2 according to the location of their somata and dendritic processes and were counted.RESULTS. Under LD cycles an increase in the number of immunoreactive cells was observed within the first week of postnatal development. When mice were maintained in DD, the increase in the number of immunopositive cells detected was significantly higher than that in LD. On the contrary, when mice were exposed to LL within the same period, no increase was detected. To determine whether the effect of LL during the early postnatal period was reversible, the authors studied animals born in LL and subsequently maintained under LD cycles. After 3 days in LD, these animals showed a significant increase in melanopsin cell number. However, after 1 month in LD, the number was similar to that of the LD controls. Surprisingly, when mice born in DD were exposed to LL, no decrease was detected, though the immunostaining was of low intensity.CONCLUSIONS. The amount of melanopsin protein per cell varies, depending on ambient light conditions. Periods of darkness or, more likely, the sequence of light and dark periods occurring under the daily cycles might be necessary for the normal development of the melanopsin system. (Invest Ophthalmol Vis Sci. 2010;51:4840 - 4847) DOI:10.1167/iovs.10-5253