Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
Bioluminescence Imaging of an Immunocompetent Animal Model for Glioblastoma
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DOI：
10.3791/53287
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发表时间：
2016-01-01
影响因子：
1.2
通讯作者：
Hashizume, Rintaro
中科院分区：
文献类型：
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作者：
Clark, Aaron J.;Fakurnejad, Shayan;Hashizume, Rintaro
与通常报道的人脑胶质瘤异种移植动物模型相比，GL261小鼠脑胶质瘤异种移植瘤几乎概括了人类疾病的所有相关临床和组织病理学特征。当GL261细胞被移植到同基因C57BL/6小鼠的脑内时，该模型具有维持完整的免疫微环境的额外优势。荧光素酶在GL261细胞中的稳定表达使非侵入性、经济高效的生物发光监测颅内肿瘤生长成为可能。我们最近已经证明，荧光素酶在GL261细胞中的表达不会影响肿瘤的生长特性、肿瘤细胞免疫调节细胞因子的表达、免疫细胞对肿瘤的侵袭，也不会影响携带颅内肿瘤的动物的总体生存。因此，GL261荧光素酶胶质瘤模型在新的化疗和免疫治疗方式的研究中是有用的。在这里，我们报道了在GL261细胞中产生稳定表达荧光素酶的技术，以及如何利用生物发光成像来研究肿瘤细胞的体外和体内生长。
In contrast to commonly reported human glioma xenograft animal models, GL261 murine glioma xenografts recapitulate nearly all relevant clinical and histopathologic features of the human disease. When GL261 cells are implanted intracranially in syngeneic C57BL/6 mice, the model has the added advantage of maintaining an intact immune microenvironment. Stable expression of luciferase in GL261 cells allows non-invasive cost effective bioluminescence monitoring of intracranial tumor growth. We have recently demonstrated that luciferase expression in GL261 cells does not affect the tumor growth properties, tumor cell immunomodulatory cytokine expression, infiltration of immune cells into the tumor, or overall survival of animals bearing the intracranial tumor. Therefore, it appears that the GL261 luciferase glioma model can be useful in the study of novel chemotherapeutic and immunotherapeutic modalities. Here we report the technique for generating stable luciferase expression in GL261 cells and how to study the in vitro and in vivo growth of the tumor cells by bioluminescence imaging.