Dietary fiber extracted from pomelo fruitlets promotes intestinal functions, both in vitro and in vivo
Dietary fiber extracted from pomelo fruitlets promotes intestinal functions, both in vitro and in vivo
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DOI：
10.1016/j.carbpol.2020.117186
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发表时间：
2021-01-15
影响因子：
11.2
通讯作者：
Ma, Lukai
中科院分区：
文献类型：
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作者：
Liu, Huifan;Zeng, Xinyue;Ma, Lukai
柚子果实含有多种活性物质，易于采集和加工。本文研究了柚子膳食纤维在体内和体外的生物学效应。柚子果实总膳食纤维（TDF）、可溶性膳食纤维（SDF）和不溶性膳食纤维（IDF）值分别为75.64 +/- 3.65%、10.10 +/- 1.39%和62.48 +/- 3.68%。鉴定出的主要单糖为鼠李糖、阿拉伯糖、半乳糖和葡萄糖。所有纤维都能清除游离的2,2'-二苯基-1-吡啶肼基自由基和还原的铁离子。纤维的持水、持油和膨胀能力延缓了葡萄糖的扩散，抑制了α -淀粉酶，并影响了胆固醇胶束的形成。在四氧嘧啶诱导的糖尿病小鼠模型中，SDF比TDF或IDF更好地改善了葡萄糖耐量，控制了血糖，降低了血清胰岛素。在高血糖小鼠中，所有类型的纤维都降低了总胆固醇含量和拟杆菌门、变形菌门和瘤胃球菌科的患病率，但增加了厚壁菌门、乳酸杆菌和普氏菌科的丰度。
Pomelo fruitlets contain various active substances that are easily collected and processed. Here, the biological effects of pomelo fruitlet dietary fiber were investigated in vivo and in vitro. Total dietary fiber (TDF), soluble dietary fiber (SDF), and insoluble dietary fiber (IDF) values of pomelo fruitlets were 75.64 +/- 3.65 %, 10.10 +/- 1.39 %, and 62.48 +/- 3.68 %, respectively. The main monosaccharides identified were rhamnose, arabinose, galactose, and glucose. All fibers scavenged free 2,2'-diphenyl-1-picrylhydrazyl radicals and reduced ferric cations. The water-holding, oil-holding, and swelling capacities of the fibers retarded glucose diffusion, inhibited alpha-amylase, and influenced cholesterol micelle formation. In a mouse model of alloxan-induced diabetes, SDF improved glucose tolerance, controlled blood glucose, and reduced serum insulin better than TDF or IDF. All fiber types decreased the total cholesterol content and the prevalence of Bacteroidetes, Proteobacteria, and Ruminococcaceae, but increased the abundance of Firmicutes, Lactobacillus, and Prevotellaceae in hyperglycemic mice.