Transcriptome profiling of brain edemas caused by influenza infection and lipopolysaccharide treatment
Transcriptome profiling of brain edemas caused by influenza infection and lipopolysaccharide treatment
复制标题
DOI：
10.1002/jmv.23801
复制
发表时间：
2014-05
影响因子：
12.7
通讯作者：
Yukihiro Kyan;Yumi Ueda;Mitsutaka Yoshida;K. Sasahara;K. Shinya
中科院分区：
文献类型：
--
作者：
Yukihiro Kyan;Yumi Ueda;Mitsutaka Yoshida;K. Sasahara;K. Shinya
在日本，由流感病毒感染引发的甲型流感病毒相关脑病经常发生在5岁及以下的儿童中。然而，甲型流感病毒相关脑病背后的机制尚未得到很好的理解。本研究使用感染甲型流感病毒并给予脂多糖治疗的小鼠建立了甲型流感病毒相关脑病样模型。结果表明，与对照组相比，模型中使用的小鼠遭受的脑水肿几乎是对照组的三倍，血清中的细胞因子水平也更高。使用基因表达谱分析，发现细胞因子相关基因在原位脑中没有上调，而已知参与血脑屏障破坏的蛋白质编码基因上调。使用基因本体对功能组进行分类揭示了术语“离子通道”、“钙振荡”和“膜转运活性”。因此，在该甲型流感病毒相关脑病模型中发现的血脑屏障破坏可假定是由于神经元组织的细胞电解质失衡以及细胞因子风暴所致。J. Med. Virol. 86：905-911，2014.© 2013威利期刊公司.
Influenza A virus‐associated encephalopathy triggered by influenza virus infection often occurs in children aged five and younger in Japan. However, the mechanisms behind Influenza A virus‐associated encephalopathy are not yet well understood. This study developed an Influenza A virus‐associated encephalopathy‐like model using mice infected with Influenza A virus and given lipopolysaccharide treatment. The results showed that the mice used in the model suffered from brain edemas nearly three times more severe, as well as having higher cytokine levels in sera compared to those of the control groups. Using gene expression profiling, cytokine‐related genes were found not to be up‐regulated in the brain in situ, while protein coding genes, which are known to be involved in blood–brain barrier disruption, were up‐regulated. Categorizing the functional groups using gene ontology revealed the terms “ion channels,” “calcium oscillation,” and “membrane transporter activities.” The blood–brain barrier disruption found in this Influenza A virus‐associated encephalopathy model can therefore be assumed to be due to a cellular electrolyte imbalance of the neuronal tissue, in addition to a cytokine storm. J. Med. Virol. 86:905–911, 2014. © 2013 Wiley Periodicals, Inc.