Repositioning Chloride Transmembrane Transporters: Transport of Organic Ion Pairs
Repositioning Chloride Transmembrane Transporters: Transport of Organic Ion Pairs
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DOI：
10.1002/anie.201900818
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发表时间：
2019-05-20
影响因子：
16.6
通讯作者：
Bartik, Kristin
中科院分区：
文献类型：
--
作者：
Grauwels, Glenn;Valkenier, Hennie;Bartik, Kristin
鉴于有机阳离子的生物学重要性，促进有机离子对的运输可以找到许多应用。杯状[6]芳烃三（硫）脲具有可容纳伯胺离子的空腔，不仅可以作为Cl-/NO3-反端口的载体，还可以进行PrNH3Cl的共输运。通过荧光光谱监测转运情况，并通过H-1和Cl-35核磁共振实验（含移位试剂）表征囊泡内不同物质的存在。没有空腔的受体也可以观察到PrNH3Cl的共转运，但空腔的存在传递了一个优势，因为通过杯状[6]芳烃的共转运被观察到比Cl-/NO3-反转运更有效，而没有空腔的受体则不是这样。当使用体积较大的铵离子时，这种优势的消失进一步突出了空腔所起的作用，因为铵离子不能在空腔内络合。
Given the biological importance of organic cations, the facilitated transport of organic ion pairs could find many applications. Calix[6]arene tris(thio)ureas, which possess a cavity that can accommodate primary ammonium ions, can not only act as carriers for Cl-/NO3- antiport but can also perform the cotransport of PrNH3Cl. Transport was monitored by fluorescence spectroscopy and the presence of the different species inside the vesicles was characterized by H-1 and Cl-35 NMR experiments involving shift reagents. The cotransport of PrNH3Cl was also observed by receptors deprived of a cavity, but the presence of the cavity conveys an advantage, as the cotransport by calix[6]arenes was observed to be more efficient than the Cl-/NO3- antiport, which is not the case with receptors without a cavity. The role played by the cavity was further highlighted by the disappearance of this advantage when using a bulky ammonium ion, which cannot be complexed within the cavity.