Subpopulations of Macrophages within Eutopic Endometrium of Endometriosis Patients
Subpopulations of Macrophages within Eutopic Endometrium of Endometriosis Patients
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DOI：
10.1111/aji.12331
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发表时间：
2015-03-01
影响因子：
3.6
通讯作者：
Murakami, Takashi
中科院分区：
文献类型：
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作者：
Takebayashi, Akie;Kimura, Fuminori;Murakami, Takashi
问题：子宫内膜异位症被认为是一种慢性炎症性疾病，与免疫反应有关。已有报道揭示了子宫内膜异位症患者异位子宫内膜内巨噬细胞的不同分布。巨噬细胞在功能上分化为M1和M2细胞系。我们研究了有或无子宫内膜异位症患者异位子宫内膜内M1和M2巨噬细胞亚群的差异，以研究异位子宫内膜如何受到免疫刺激。方法对36例子宫内膜异位症患者（子宫内膜异位症组）和37例无子宫内膜异位症患者（非子宫内膜异位症组）进行分析。研究采用石蜡包埋子宫内膜标本。连续切片对CD68（泛巨噬细胞标志物）和CD163 （M2巨噬细胞标志物）进行免疫染色。定量各标志物阳性的细胞，计算M2巨噬细胞占泛巨噬细胞的比例。结果子宫内膜异位症组各期泛巨噬细胞数量均显著高于对照组(P
ProblemEndometriosis is recognized as a chronic inflammatory disease and is related to immune response. There have been reports that revealed the different distribution of macrophage within the eutopic endometrium of women with endometriosis. Macrophages are functionally polarized into M1 and M2 cell lineages. We studied a difference in the subpopulations of M1 and M2 macrophages within the eutopic endometrium in patients with or without endometriosis to investigate how the eutopic endometrium is stimulated immunologically.Method of studyThirty-six patients with endometriosis (endometriosis group) and 37 without endometriosis (non-endometriosis group) were analyzed. Paraffin-embedded endometrial specimens were used for the study. Consecutive sections were used for immunostaining of CD68 (pan-macrophage marker) and CD163 (M2 macrophage marker). Cells positive for each marker were quantified, and the ratio of M2 macrophages in pan-macrophages was calculated.ResultThe endometriosis group had a significantly higher number of pan-macrophages than the control group in all phases (P