MURINE CYTOMEGALOVIRUS INDUCED CONGENITAL-DEFECTS AND FETAL MALDEVELOPMENT
MURINE CYTOMEGALOVIRUS INDUCED CONGENITAL-DEFECTS AND FETAL MALDEVELOPMENT
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DOI：
10.1093/infdis/148.5.836
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发表时间：
1983-01-01
影响因子：
6.4
通讯作者：
HUANG, ES
中科院分区：
文献类型：
--
作者：
BASKAR, JF;STANAT, SC;HUANG, ES
为探讨先天性巨细胞病毒感染对胚胎发育的影响，将小鼠巨细胞病毒(MCMV)注入妊娠早期小鼠子宫内膜腔。随后处死小鼠，并检查胚胎着床的部位。在感染MCMV的动物中，产仔数显著减少，异常胎儿的发生率显著增加。发现了病态、奄奄一息、严重发育迟缓、发育不同步和畸形的胎儿。病毒是从感染的母亲、胎盘和胎儿组织中重新分离出来的，而不是从接受了热灭活病毒或培养液的对照动物身上分离出来的。经限制性内切酶分析证实，回收的病毒与原病毒完全一致。用原位核酸杂交法在实验组织切片中检测到MCMV特异性DNA。感染的雌性小鼠出现血清转换。因此，胚胎植入时生殖道中MCMV的存在不仅会导致胚胎感染，还会导致胎儿发育不良。
The effects of congenital infection with cytomegalovirus on fetal development were studied by injection of murine cytomegalovirus (MCMV) into the endometrial lumina of mice in the early stages of pregnancy. The mice were later killed, and the sites of embryonic implantation were examined. Litter size was reduced and the incidence of abnormal fetuses was significantly increased among MCMV-infected animals. Morbid, moribund, severely growth-retarded, asynchronously developed and malformed fetuses were found. Virus was reisolated from infected mothers, placentas, and fetal tissues but not from control animals that had received either heat-inactivated virus or culture medium. The recovered virus was identical to the original virus, as verified by restriction enzyme analysis. MCMV-specific DNA was detected in experimental tissue sections by in situ nuclei-acid hybridization. Seroconversion occurred in infected female mice. Thus, the presence of MCMV in the genital tract at the time of embryonic implantation causes not only embryonic infection but also fetal maldevelopment.