Analysis of polycyclic aromatic hydrocarbons in fish: evaluation of a quick, easy, cheap, effective, rugged, and safe extraction method
Analysis of polycyclic aromatic hydrocarbons in fish: evaluation of a quick, easy, cheap, effective, rugged, and safe extraction method
复制标题
DOI：
10.1002/jssc.200900351
复制
发表时间：
2009-10-01
影响因子：
3.1
通讯作者：
Prior Pinto Oliveira, Maria Beatriz
中科院分区：
文献类型：
--
作者：
Ramalhosa, Maria Joao;Paiga, Paula;Prior Pinto Oliveira, Maria Beatriz
对 QuEChERS 方法从鱼类样品中提取 16 种多环芳烃进行了评估。为了选择性测量化合物，通过 LC 和荧光检测对提取物进行分析。整个分析程序通过三个水平的系统回收实验和使用标准参考材料 SRM 2977（贻贝组织）进行了验证。从SRM 2977中成功提取了除萘和苊之外的目标污染物，回收率范围为63.5-110.0%，变异系数不超过8%。最佳 QuEChERS 条件如下：5 g 均质鱼样品，10 ml ACN，涡旋搅拌 3 分钟。获得的定量限范围为 0.12-1.90 ng/g 湿重 (0.30-4.70 mu g/L)。应用优化的方法评估竹荚鱼（Trachurus trachurus）、鲭鱼（Scomber japonicus）、沙丁鱼（Sardina pilchardus）和养殖鲈鱼（Dicentrarchus labrax）PAHs含量的安全性。尽管用于评估食品中多环芳烃致癌风险的标志物苯并(a)芘在分析样本中未检出（89 个体对应 27 个均质样本），但总体平均浓度范围为竹荚鱼中的 2.52 +/- 1.20 ng/g 到养殖鲈鱼中的 14.6 +/- 2.8 ng/g。四组的平均 PAH 浓度之间存在显着差异。
QuEChERS method was evaluated for extraction of 16 PAHs from fish samples. For a selective measurement of the compounds, extracts were analysed by LC with fluorescence detection. The overall analytical procedure was validated by systematic recovery experiments at three levels and by using the standard reference material SRM 2977 (mussel tissue). The targeted contaminants, except naphthalene and acenaphthene, were successfully extracted from SRM 2977 with recoveries ranging from 63.5-110.0% with variation coefficients not exceeding 8%. The optimum QuEChERS conditions were the following: 5 g of homogenised fish sample, 10 ml, of ACN, agitation performed by vortex during 3 min. Quantification limits ranging from 0.12-1.90 ng/g wet weight (0.30-4.70 mu g/L) were obtained. The optimized methodology was applied to assess the safety concerning PAHs contents of horse mackerel (Trachurus trachurus), chub mackerel (Scomber japonicus), sardine (Sardina pilchardus) and farmed seabass (Dicentrarchus labrax). Although benzo(a)pyrene, the marker used for evaluating the carcinogenic risk of PAHs in food, was not detected in the analysed samples (89 individuals corresponding to 27 homogenized samples), the overall mean concentration ranged from 2.52 +/- 1.20 ng/g in horse mackerel to 14.6 +/- 2.8 ng/g in farmed seabass. Significant differences were found between the mean PAHs concentrations of the four groups.