KIF2Aβ:: A kinesin family member enriched in mouse male germ cells, interacts with Translin associated factor-X (TRAX)
KIF2Aβ:: A kinesin family member enriched in mouse male germ cells, interacts with Translin associated factor-X (TRAX)
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DOI：
10.1002/mrd.20171
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发表时间：
2004-12-01
影响因子：
2.5
通讯作者：
Hecht, NB
中科院分区：
文献类型：
--
作者：
Bray, JD;Chennathukuzhi, VM;Hecht, NB
Translin 相关因子 X (TRAX) 是 TB-RBP/Translin 的结合伴侣。在酵母双杂交文库筛选中从小鼠睾丸 cDNA 中分离出编码 KIF2Abeta 260 个 C 端氨基酸的 cDNA，以筛选特定的 TRAX 相互作用蛋白。 KIF2Abeta 主要在小鼠睾丸中表达，并在生殖细胞中富集。使用体外合成的融合蛋白验证了全长 KIF2Abeta 或其 C 末端与 TRAX 的相互作用。 KIF2Abeta TRAX 结合区的缺失图谱表明，氨基酸 514-659 对于体内相互作用是必要且充分的。使用 GFP 融合蛋白的共聚焦显微镜研究表明，KIF2Abeta 与 TRAX 共定位于核周位置。 KIF2Abeta 不与 TB-RBP 相互作用，表明 TRAX 可以作为运动蛋白和 TB-RBP 的衔接分子，或者这种相互作用揭示了 TRAX 在生殖细胞中的未描述的作用。与 KIF2Abeta 的相互作用表明 TRAX 在精子发生过程中基于微管的功能中发挥作用。 (C) 2004 Wiley-Liss, Inc.
Translin associated factor X (TRAX) is a binding partner of TB-RBP/Translin. A cDNA encoding the 260 C-terminal amino acids of KIF2Abeta was isolated from mouse testis cDNAs in a yeast two-hybrid library screen for specific TRAX-interacting proteins. KIF2Abeta was expressed predominantly in the mouse testis and enriched in germ cells. The interaction of full-length KIF2Abeta or its C-terminus with TRAX was verified using in vitro synthesized fusion proteins. Deletion mapping of the TRAX-binding region of KIF2Abeta indicated that amino acids 514-659 were necessary and sufficient for the interaction in vivo. Confocal microscopy studies using GFP-fusion proteins demonstrated that KIF2Abeta colocalizes with TRAX in a perinuclear location. KIF2Abeta does not interact with TB-RBP, suggesting that either TRAX can function as an adaptor molecule for motor proteins and TB-RBP, or that this interaction reveals an undescribed role for TRAX in germ cells. The interaction with KIF2Abeta suggests a role for TRAX in microtubule-based functions during spermatogenesis. (C) 2004 Wiley-Liss, Inc.