MED26‐containing Mediator may orchestrate multiple transcription processes through organization of nuclear bodies
MED26‐containing Mediator may orchestrate multiple transcription processes through organization of nuclear bodies
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DOI：
10.1002/bies.202200178
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发表时间：
2023-02
期刊：
影响因子：
4
通讯作者：
Hidefumi Suzuki;Kazuki Furugori;Ryota Abe;Shintaro Ogawa;Sayaka Ito;Tomohiko Akiyama;Keiko Horiuchi;Hidehisa Takahashi
中科院分区：
文献类型：
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作者：
Hidefumi Suzuki;Kazuki Furugori;Ryota Abe;Shintaro Ogawa;Sayaka Ito;Tomohiko Akiyama;Keiko Horiuchi;Hidehisa Takahashi
介体是一种协同调节复合体，在多种转录调控过程中发挥着重要作用。MED26是人类介体亚基之一，其作用是将超伸长复合体(SEC)募集到多聚腺化基因，将小延伸复合体(LEC)募集到非多腺化基因，包括小核RNAs(SnRNAs)和复制依赖组蛋白(RDH)基因。含有MED26的介体通过Cajal小体(CBS)向组蛋白小体(HLB)的募集，在3‘Pol II转录末端区域的停顿中发挥作用。这一发现表明，Mediator参与了CBS与HLBS的联系，通过提供CBS的3‘端加工因子，促进了3’Pol II暂停和随后的3‘端加工。因此，我们认为，Mediator参与了核体的组织，从而协调了基因转录的多个过程。
Mediator is a coregulatory complex that plays essential roles in multiple processes of transcription regulation. One of the human Mediator subunits, MED26, has a role in recruitment of the super elongation complex (SEC) to polyadenylated genes and little elongation complex (LEC) to non‐polyadenylated genes, including small nuclear RNAs (snRNAs) and replication‐dependent histone (RDH) genes. MED26‐containing Mediator plays a role in 3′ Pol II pausing at the proximal region of transcript end sites in RDH genes through recruitment of Cajal bodies (CBs) to histone locus bodies (HLBs). This finding suggests that Mediator is involved in the association of CBs with HLBs to facilitate 3′ Pol II pausing and subsequent 3′‐end processing by supplying 3′‐end processing factors from CBs. Thus, we argue the possibility that Mediator is involved in the organization of nuclear bodies to orchestrate multiple processes of gene transcription.