Effects of specific gangliosides on the in vitro proliferation of MPTP-susceptible cells.
Effects of specific gangliosides on the in vitro proliferation of MPTP-susceptible cells.
复制标题
特定神经节苷脂对 MPTP 敏感细胞体外增殖的影响。
DOI：
10.1111/j.1471-4159.1993.tb13619.x
复制
发表时间：
1993
影响因子：
4.7
通讯作者：
Schengrund,CL
中科院分区：
文献类型：
--
作者：
Saulino,MF;Schengrund,CL
为了确定神经节苷脂分子的哪一部分对于增强MPTP诱导的损伤的恢复可能是必需的，研究了特定神经节苷脂刺激MPTP处理的140-3细胞增殖的能力。结果表明，在所测试的神经节苷脂中，GM 1是最有效的。尽管GD 1 a和GT 1 B能够增强MPTP处理的细胞的增殖，但需要两倍的GT 1 B来诱导与GM 1相同的效果。相比之下，去唾液酸-GM 1、GM 2和GM 3在促进MPTP处理的细胞的增殖方面无效。而GM 1的分离寡糖则无明显作用。这些结果表明，除唾液酸残基外，至少寡糖链的Gal（β1-3）Gal-NAc部分和神经酰胺部分对于诱导MPTP处理的细胞增殖是必需的。对添加GM 1的时间对其抵消MPTP诱导的140-3细胞增殖抑制的能力的研究表明，在MPTP之前或与MPTP同时添加GM 1导致MPTP诱导的细胞增殖抑制显著降低。
To determine which portion of a ganglioside molecule might be necessary for the enhancement of recovery from MPTP‐induced lesions, the ability of specific gangliosides to stimulate proliferation of MPTP‐treated 140–3 cells was investigated. The results indicate that of the gangliosides tested, GM1 was the most effective. Although GD1 a and GT1 b were able to enhance the proliferation of MPTP‐treated cells, twice as much GT1b was needed to induce the same effect seen with GM1. In contrast, asialo‐GM1, GM2, and GM3 were ineffective at promoting proliferation of MPTP‐treated cells. The isolated oligosaccharide of GM1 had little effect. These results indicate that in addition to the sialosyl residue, at least the Gal(β1–3)Gal‐NAc portion of the oligosaccharide chain and the ceramide moiety are essential for the induction of proliferation of the MPTP‐treated cells. Investigation of the time of addition of GM1 on its ability to counteract the MPTP‐induced inhibition of 140–3 cell proliferation indicated that addition of GM1 before or concomitantly with MPTP resulted in a significant reduction in MPTP‐induced inhibition of cell proliferation.