Identification of sulfoglycolipid bioactivities and characteristic fatty acids of marine macroalgae.
Identification of sulfoglycolipid bioactivities and characteristic fatty acids of marine macroalgae.
复制标题
DOI：
10.1021/jf302241d
复制
发表时间：
2012-08
影响因子：
6.1
通讯作者：
Cheng-Jung Tsai;Bonnie Sun Pan
中科院分区：
文献类型：
--
作者：
Cheng-Jung Tsai;Bonnie Sun Pan
摘要本研究采自台湾东北部海域，采自绿藻门（绿色藻）5种、红藻门（红藻）13种、异鞭毛藻门（褐藻）3种共21种大型海藻，分析其脂肪酸组成，以探讨其功能性脂质。绿色藻类的脂质含量范围为15.36 - 20.15 mg/g（干基），其特征在于C18：2和C18：3含量高，红藻（18.57-28.34 mg/g db）在C20：4和C20：5中含量高，褐藻（13.11-19.56 mg/g db）在C18：4、C20：4和C：20：5中含量高。所有的藻类脂质都含有奇数碳的脂肪酸，C17：0和C17：1。红藻的多不饱和脂肪酸（PUFAs）含量相对较高，二十碳五烯酸（EPA）含量高于绿色和褐藻。用乙醇提取卷曲紫菜中的硫代糖脂，经Diaion HP-20柱分离，得到硫代喹诺糖基甘油二酯（sulfoquinovosyldiacylglycerols，SQDG）。SQDG中主要脂肪酸为棕榈酸（C16：0）、EPA（C20：5）、花生四烯酸（C20：4）、油酸（C18：1）和硬脂酸（C18：0），分别占33.3%、30.0%、12.7%、7.52%和6.83%。n-3/n-6的比例为1.9，而真正的标准，菠菜SQDG，不含n-3脂肪酸。磺基糖脂对人肝癌细胞株HepG 2的生长有抑制作用。IC 50为126 μg/mL，低于菠菜SQDG（255 μg/mL）。
The fatty acid compositions of 21 species of marine macroalgae, including 5 species of Chlorophyta (green algae), 13 of Rhodophyta (red algae), and 3 of Heterokontophyta (brown algae), were collected from northeastern Taiwan to survey their functional lipids. The lipid contents of green algae ranged from 15.36 to 20.15 mg/g, dry basis (db), and were characterized by a high content of C18:2 and C18:3, red algae (18.57-28.34 mg/g db) were high in C20:4 and C20:5, and brown algae (13.11-19.56 mg/g db) were high in C18:4, C20:4, and C:20:5. All algal lipids contained fatty acids of odd-number carbons, C17:0, and C17:1. Red algae had relatively higher levels of polyunsaturated fatty acids (PUFAs) and were richer in eicosapentaenoic acid (EPA) than green and brown algae. A red alga, Porphyra crispata , was extracted with ethanol and separated on a hydrophobic column (Diaion HP-20 column) to obtain sulfoglycolipids (sulfoquinovosyldiacylglycerols, SQDGs). The main fatty acids in SQDGs were palmitic acid (C16:0), 33.3%; EPA (C20:5), 30.0%; arachidonic acid (C20:4), 12.7%; oleic acid (C18:1), 7.52%; and stearic acid (C18:0), 6.83%. The n-3/n-6 ratio was 1.9, whereas the authentic standard, spinach SQDG, did not contain n-3 fatty acids. Sulfoglycolipids inhibited the growth of human hepatocellular carcinoma cell line (HepG2). The IC50 was 126 μg/mL, which is lower than that of the spinach SQDG (255 μg/mL).