Cultivation and Functional Characterization of a Deep-Sea Lentisphaerae Representative Reveals Its Unique Physiology and Ecology
Cultivation and Functional Characterization of a Deep-Sea Lentisphaerae Representative Reveals Its Unique Physiology and Ecology
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深海 Lentisphaerae 代表的培养和功能表征揭示了其独特的生理学和生态学
DOI：
10.3389/fmars.2022.848136
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发表时间：
2022
影响因子：
1.7
通讯作者：
Chaomin Sun
中科院分区：
文献类型：
--
作者：
Tian;Rikuan Zheng;Rui Liu;Ronggui Li;Chaomin Sun
作为浮游菌-疣微菌-衣原体（Planctomycetes-Verrucomicrobia-Chlamydia，PVC）超门的一员，慢球菌门广泛分布于各种环境中。慢球酵母属的成员被认为是典型的难以在纯培养中分离的，迄今为止只有五个属于该门的菌株被培养，因此它们的主要生理学和生态学在很大程度上是未知的。在这里，使用多糖降解驱动的策略，我们成功地培养了一个代表性的Lentisphaerobic细菌（Lentisphaerobic Coldseepensis marina zth 2）从深海冷泉。根据生理学和系统发育分析，菌株zth 2被鉴定为一个新家族的模式菌株。值得注意的是，菌株zth 2以出芽方式分裂，并且在超薄切片电子显微镜观察中看到的独特特征是在菌株zth 2的表面上出现窄突起，即细胞壁和细胞质。通过生长测定和转录组学分析，证实菌株zth 2有效地利用多糖、磷酸盐和铁用于生长。引人注目的是，多糖、磷酸盐和铁的利用是相关的，并且共同有助于碳、磷和铁的代谢，从而促进能量的产生。通过模拟海洋环境并使用操作分类单位（OTUs）分析，我们证明菌株zth 2通过代谢多糖或其他物质显著丰富微生物多样性。总的来说，我们建议，Lentisphaesthetic成员是潜在的贡献者的营养物质，碳，磷和其他元素在深层生物圈的循环。
As a member of Planctomycetes–Verrucomicrobia–Chlamydia (PVC) superphylum, the phylum Lentisphaerae is broadly distributed in various environments. Lentisphaerae members have been recognized as being typically difficult to isolate in pure culture—only five strains belonging to this phylum were cultured up to now—and therefore their major physiology and ecology are largely unknown. Here, using a polysaccharide degradation-driven strategy, we successfully cultured a representative of Lentisphaerae bacteria (Candidatus Coldseepensis marina zth2) from the deep-sea cold seep. Based on physiological and phylogenetic analyses, strain zth2 is identified as a type strain of a novel family. Notably, strain zth2 divides by a budding manner and a unique feature seen in ultrathin-section electron microscopic observation is the occurrence of narrow protrusions, namely, both cell wall and cytoplasm on the surface of strain zth2. Through growth assays and transcriptomic analyses, strain zth2 was verified to efficiently utilize polysaccharides, phosphate, and iron for growth. Strikingly, the utilization of polysaccharide, phosphate, and iron is correlative and jointly contributes to carbon, phosphorus, and iron metabolisms and thereby facilitating the production of energy. By mimicking the ocean environment and using operational taxonomic units (OTUs) analysis, we demonstrated that strain zth2 significantly enrich the microbial diversity by metabolizing polysaccharides or other substances. Overall, we propose that Lentisphaerae members are potential contributors to the cycling of nutrient, carbon, phosphorus, and other elements in the deep biosphere.
DOI：
10.1073/pnas.0602047103
发表时间：
2006-08-01
影响因子：
11.1
作者：
Wagner, Jennifer K.;Setayeshgar, Sima;Brun, Yves V.
通讯作者：
Brun, Yves V.
影响因子：
7
作者：
Haas, Brian J.;Gevers, Dirk;Birren, Bruce W.
通讯作者：
Birren, Bruce W.
影响因子：
28.3
作者：
Sichert, Andreas;Corzett, Christopher H.;Hehemann, Jan-Hendrik
通讯作者：
Hehemann, Jan-Hendrik