Analysis of the entire nucleotide sequence of hepatitis B virus genotype B in the Philippines reveals a new subgenotype of genotype B
Analysis of the entire nucleotide sequence of hepatitis B virus genotype B in the Philippines reveals a new subgenotype of genotype B
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DOI：
10.1099/vir.0.81525-0
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发表时间：
2006-05-01
影响因子：
3.8
通讯作者：
Shimosegawa, T
中科院分区：
文献类型：
--
作者：
Nagasaki, F;Niitsuma, H;Shimosegawa, T
确定了从菲律宾5名患者中提取的B型肝炎病毒（HBV）基因型B（HBV/B）基因组的全部核苷酸序列，并命名为GenBank AB 219426、AB 219427、AB 219428、AB 219429和AB 219430。前4个分离株的血清型均为ayw，GenBank AB 219430的血清型均为adw。前4个分离物的全序列与GenBank A132119430的全序列的差异为1.0-2.0%，与GenBank A132119430的全序列的差异为3.8- 4.2%。系统发育树分析显示，在世界范围内，HBV/B包括五个亚型：1311，82，133，B4和新的菲律宾组，指定135。B5亚型中的4个分离株全基因组序列与其他国家（亚型）分离株的差异分别为：越南（E14）4.4- 4.8%、印度尼西亚（B3）2.9- 3.5%、中国（B2）4.7- 5.1%、日本（B1）5.4-6.0%。与此类似，GenBank A8219430显示出最低的差异：与印度尼西亚分离株（B3）的差异为3.4%，与越南分离株（B4）的差异为5.0%，与中国分离株（B2）的差异为5.4%，与日本分离株（B1）的差异为6.1%。这是首次报道菲律宾HBV/B的全核苷酸序列，结果表明这些序列属于一个新的亚型B5。本研究发现，世界各地的HBV/B分离株在遗传上可分为五个亚型，地理距离与HBV/B的遗传距离之间的关系具有良好的相关性。
The entire nucleotide sequences were determined for hepatitis B virus (HBV) genotype B (HBV/B) genomes extracted from five patients in the Philippines and designated GenBank AB219426, AB219427, AB219428, AB219429 and AB219430. The serotype of the first four isolates was ayw and that of GenBank AB219430 was adw. Divergences of entire sequences were 1.0-2.0% between the first four isolates and 3.8-4.2 % between these four and GenBank A132119430. Phylogenetic-tree analysis revealed that, worldwide, HBV/B comprises five subgenotypes: 1311, 82, 133, B4 and the new Philippines group, designated 135. Divergences of the entire genome sequences between four isolates in subgenotype B5 and isolates from other countries (subgenotypes) were 4.4-4.8 % with Vietnam (E14), 2.9-3.5 % with Indonesia (B3), 4.7-5.1 % with China (B2) and 5.4-6.0% with Japan (B1). Similarly, GenBank A8219430 showed the lowest divergences: 3.4 % with the isolate from Indonesia (B3), 5.0 % with Vietnam (B4), 5.4 % with China (B2) and 6.1 % with Japan (B1). This is the first report of entire nucleotide sequences of HBV/B from the Philippines and the results show that these sequences belong to a new subgenotype, B5. The present study identified that HBV/B isolates throughout the world are divided genetically into five subgenotypes, the relationships between geographical distances and the genetic distances of HBV/B being well-correlated.