Expression of the regulatory protein LndI for landomycin E production in Streptomyces globisporus 1912 is controlled by the availability of tRNA for the rare UUA codon
Expression of the regulatory protein LndI for landomycin E production in Streptomyces globisporus 1912 is controlled by the availability of tRNA for the rare UUA codon
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DOI：
10.1111/j.1574-6968.2005.00087.x
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发表时间：
2006-03-01
影响因子：
2.1
通讯作者：
Fedorenko, VO
中科院分区：
文献类型：
--
作者：
Rebets, YV;Ostash, BO;Fedorenko, VO
lndI基因编码陆霉素生物合成激活因子。利用lndI - egfp融合，我们研究了该基因表达的时间模式，并证明了lndI转录和翻译之间的延迟。lndI中的TTA密码子被认为是造成这种延迟的原因。用CTC代替TTA消除了lndI转录和翻译之间的停顿。lndI基因的野生型在colicolor链霉菌bldA（-）突变株中未表达，说明bldA tRNA在其mRNA翻译中的重要性。
The gene lndI encodes the activator of landomycin biosynthesis. The utilization of LndI-EGFP fusions led us to investigate the temporal pattern of this gene expression and demonstrated the delay between lndI transcription and translation. The TTA codon in lndI is thought to be the reason for this delay. The replacement of TTA with CTC cancelled the pause between lndI transcription and the translation. The wild-type of the lndI gene is not expressed in the Streptomyces coelicolor bldA(-) mutant strain, indicating the importance of the bldA tRNA in its mRNA translation.