Borrelia burgdorferi BmpA, BmpB, and BmpD proteins are expressed in human infection and contribute to P39 immunoblot reactivity in patients with Lyme disease
Borrelia burgdorferi BmpA, BmpB, and BmpD proteins are expressed in human infection and contribute to P39 immunoblot reactivity in patients with Lyme disease
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DOI：
10.1128/cdli.12.8.935-940.2005
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发表时间：
2005-08-01
期刊：
影响因子：
--
通讯作者：
Cabello, FC
中科院分区：
文献类型：
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作者：
Bryksin, AV;Godfrey, HP;Cabello, FC
Bmp蛋白是一个同源家族的染色体编码伯氏疏螺旋体脂蛋白。在莱姆病聚丙烯酰胺凝胶电泳免疫印迹中，它们具有相似的预测免疫原性和相似的十二烷基硫酸钠裂解物的电泳迁移率。长期以来，P39对伯氏疏螺旋体病患者的反应性一直被确定为对BmpA的反应性，但对其他Bmp蛋白的反应尚未被检测。为了确定莱姆病患者是否会出现这种反应，我们利用大肠杆菌中表达的可溶性重组Bmp （rBmp）蛋白，研究了患者和对照血清中免疫球蛋白G （IgG）抗Bmp的反应性。尽管一些患者的血清中含有针对所有四种Bmp蛋白的IgG免疫印迹和免疫点反应性，但通过分级剂量的可溶性同源和异源rBmp蛋白的竞争性酶联免疫吸附试验分析IgG抗Bmp精细特异性表明，只有对BmpA， BmpB和BmpD的反应是特异性的。这表明，在感染患者的四种弧菌中至少有三种，针对它们的特异性抗体可能是Bmp蛋白，这些蛋白由伯氏疏螺旋体表达，存在于一些患者的P39带中。
The Bmp proteins are a paralogous family of chromosomally encoded Borrelia burgdorferi lipoproteins. They have similar predicted immunogenicities and similar electrophoretic mobilities by sodium dodecyl sulfate lysate in immunoblots of Lyme polyacrylamide gel electrophoresis. P39 reactivity against Borrelia burgdorferi disease patients has long been identified with reactivity to BmpA, but responses to other Bmp proteins have not been examined. To determine if patients with Lyme disease developed such responses, immunoglobulin G (IgG) anti-Bmp reactivity in patient and control sera was studied by using soluble recombinant Bmp (rBmp) proteins expressed in Escherichia coli. Although some patient sera contained IgG immunoblot and immunodot reactivities against all four Bmp proteins, analysis of IgG anti-Bmp fine specificity by a competitive enzymelinked inummosorbent assay with graded doses of soluble homologous and heterologous rBmp proteins showed that only the responses to BmpA, BmpB, and BmpD were specific. This suggests that at least three of the four fieri in infected patients and that specific antibodies to them are likely Bmp proteins are expressed by B. burgdorferi to be present in the P39 band in some patients.