Magnetic labeling of non-phagocytic adherent cells with iron oxide nanoparticles: a comprehensive study
Magnetic labeling of non-phagocytic adherent cells with iron oxide nanoparticles: a comprehensive study
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DOI：
10.1002/cmmi.256
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发表时间：
2008-11-01
影响因子：
--
通讯作者：
Muller, Robert N.
中科院分区：
文献类型：
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作者：
Boutry, Sebastien;Brunin, Stephanie;Muller, Robert N.
小颗粒氧化铁（SPIO）和超小颗粒氧化铁（USPIO），在T-2和T-2* 加权MR图像上诱导强烈的负对比度，是最常用的系统，用于培养细胞的磁标记和随后的磁共振成像（MRI）检测。本工作的目的是研究铁孵育浓度，纳米粒子的大小和纳米粒子涂层的磁性标记和非吞噬贴壁细胞在培养中的活力的影响。采用4.7T磁共振T2加权成像和基于Perl普鲁士蓝染色的铁剂量或细胞化学显示方法研究了3 T6成纤维细胞的磁标记。将细胞与增加铁浓度的SPIO（25-1000 μ g Fe/ml）一起温育48小时。将Sinerem（R），一种涂有中性葡聚糖的USPIO（20-40 nm），和Resovist（R）（65 nm），一种带有阴离子羧基葡聚糖涂层的SPIO，与作为磁性标签的Endorem（R）（涂有葡聚糖，80-150 nm）进行比较。将从大鼠股骨分离的骨髓基质细胞原代培养物（MSC）的铁负荷与3 T6成纤维细胞的铁负荷进行比较。发现用Endorem（R）标记细胞的SPIO依赖于铁孵育浓度。MSC，更稀疏地分布在文化中，表现出更高的铁含量比更密集的3 T6成纤维细胞培养。用Resovist（R）比用Endorem（R）实现更大的铁负载，Endorem（R）作为磁性标签又比Sinerem（R）更有效。因此，发现用氧化铁纳米颗粒对培养的非吞噬粘附细胞进行磁性标记取决于磁性标签和培养物中细胞的相对浓度、纳米颗粒尺寸和涂层类型。通过评估细胞膜渗透性的方法估计的细胞活力在本工作中使用的条件下不受磁性标记的影响。版权所有（C）2008约翰威利父子有限公司
Small particles of iron oxide (SPIO) and ultrasmall particles of iron oxide (USPIO), inducing a strong negative contrast on T-2 and T-2*-weighted MR images, are the most commonly used systems for the magnetic labeling of cultured cells and their subsequent detection by magnetic resonance imaging (MRI). The purpose of this work is to study the influence of iron incubation concentration, nanoparticle size and nanoparticle coating on the magnetic labeling and the viability of non-phagocytic adherent cells in culture. The magnetic labeling of 3T6 fibroblasts was studied by T-2-weighted MRI at 4.7T and by dosing-or cytochemical revealing-of iron through methods based on Perl's Prussian blue staining. Cells were incubated for 48 h with increasing iron concentrations of SPIO (25-1000 mu g Fe/ml Endorem(R)). Sinerem(R), a USPIO (20-40 nm) coated with neutral dextran, and Resovist(R) (65 nm), a SPIO bearing an anionic carboxydextran coating, were compared with Endorem(R) (dextran-coated, 80-150 nm) as magnetic tags. The iron loading of marrow stromal cell primary cultures (MSCs) isolated from rat femurs was compared with that of 3T6 fibroblasts. The SPIO-labeling of cells with Endorem(R) was found to be dependent on the iron incubation concentration. MSCs, more sparsely distributed in the culture, exhibited higher iron contents than more densely populated 3T6 fibroblast cultures. A larger iron loading was achieved with Resovist(R) than with Endorem(R), which in turn was more efficient than Sinerem(R) as a magnetic tag. The magnetic labeling of cultured non-phagocytic adherent cells with iron oxide nanoparticles was thus found to be dependent on the relative concentration of the magnetic tag and of the cells in culture, on the nanoparticle size, and on the coating type. The viability of cells, estimated by methods assessing cell membrane permeability, was not affected by magnetic labeling in the conditions used in this work. Copyright (C) 2008 John Wiley & Sons, Ltd.