Organization and stability of endogenous xenotropic murine leukemia virus proviral DNA in mouse genomes
Organization and stability of endogenous xenotropic murine leukemia virus proviral DNA in mouse genomes
复制标题
小鼠基因组中内源性异种鼠白血病病毒原病毒 DNA 的组织和稳定性
作者：
M. Hoggan;C. Buckler;J. Sears;W. Rowe;M. Martin
在一系列的印迹杂交实验中，使用异嗜性包膜探针和限制性内切酶已知的异嗜性前病毒DNA的单一时间（EcoRI）或根本没有（HindIII），我们研究了内源性异嗜性env相关序列在各种小鼠品系的组织和关系。在用HindIII或EcoRI消化后，在所有小鼠DNA中发现多个拷贝（18至28个）的异嗜性env反应性片段，并且大多数片段的大小与其来自全长前病毒DNA的来源相容。五HindIII和五EcoRI限制性片段是共同的所有近交系小鼠DNA测试。此外，每个菌株都表现出独特的异嗜性环境反应条带;这些条带在多年的近亲繁殖过程中非常稳定。每种品系特有的卵裂模式也可用于显示各种近交系小鼠之间的亲缘关系。
In a series of blot hybridization experiments, using a xenotropic envelope probe and restriction enzymes known to cut xenotropic proviral DNA a single time (EcoRI) or not at all (HindIII), we have studied the organization and relationship of endogenous xenotropic env-related sequences in various mouse strains. Multiple copies (18 to 28) of xenotropic env-reactive fragments were found in all mouse DNAs after digestion with either HindIII or EcoRI, and the majority of fragments were of sizes compatible with their origin from full-length proviral DNA. Five HindIII and five EcoRI restriction fragments were common to all inbred mouse DNAs tested. In addition, each strain exhibited unique characteristic xenotropic env-reactive bands; these bands were remarkably stable during many years of inbreeding. The cleavage patterns characteristic of each strain were also useful for showing genealogical relatedness among the various inbred mice.