Evidence for a Minor Gene-for-Minor Gene Interaction Explaining Nonhypersensitive Polygenic Partial Disease Resistance
Evidence for a Minor Gene-for-Minor Gene Interaction Explaining Nonhypersensitive Polygenic Partial Disease Resistance
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DOI：
10.1094/phyto-03-12-0056-r
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发表时间：
2012-11-01
期刊：
影响因子：
3.2
通讯作者：
Niks, Rients E.
中科院分区：
文献类型：
--
作者：
Gonzalez, Ana M.;Marcel, Thierry C.;Niks, Rients E.
部分耐药是一种定量耐药类型，根据Parlevliet的定义，不基于超敏反应。它在很大程度上是非特异性的致病型，虽然有一些小的分离株特异性反应的报告。为了阐明部分抗性基因的分离物特异性，分析了3个大麦重组自交系作图群体对叶锈菌的抗性。用抗病基因Rph 7 g的1个无毒菌株和2个毒力菌株接种作图群体。检测到6个显著的数量性状基因座（QTL）。其中，仅用无毒力分离株（1.2.1.）1株（Rphq 18）仅与两个强毒分离株（CO-04和28.1）结合。这些QTL的有效性进行了测试，与14个菌株，使用测试器组的基因型含有这些QTL的抗性或易感性的等位基因。QTL Rphq 18仅对CO-04和28.1两个菌株有效。而Rphq 3和Rphq 11对CO-04和28.1无效，但对除一个菌株外的所有菌株有效。这导致了显著的Person差异相互作用，这是基因对基因相互作用的标志。次要基因相互作用的次要基因不是基于超敏反应，也没有证据表明耐药性是基于属于核苷酸结合富亮氨酸重复序列类的基因。
Partial resistance is a quantitative type of resistance that, by definition of Parlevliet, is not based on hypersensitivity. It is largely pathotype nonspecific, although some minor isolate-specific responses have been reported. In order to elucidate the isolate specificity of individual genes for partial resistance, three barley recombinant inbred line mapping populations were analyzed for resistance to the leaf rust fungus Puccinia hordei. The mapping populations were inoculated with one isolate avirulent and two isolates virulent to resistance gene Rph7g. Six significant quantitative trait loci (QTLs) were detected. Of these, two (Rphq3 and Rphq11) were detected with only the avirulent isolate (1.2.1.) and one (Rphq18) only with both virulent isolates (CO-04 and 28.1). The effectiveness of these QTLs was tested with 14 isolates, using a tester set of genotypes containing alleles for resistance or susceptibility for these QTLs. QTL Rphq18 was effective to only two isolates, CO-04 and 28.1. whereas Rphq3 and Rphq11 were ineffective to CO-04 and 28.1 but effective to all other isolates, except one. This resulted in a significant Person's differential interaction, which is a hallmark of a gene for gene interaction. The minor gene for minor gene interaction is not based on hypersensitivity and there is no evidence that the resistance is based on genes belonging to the nucleotide-binding leucine-rich repeat class.