FLUORIMETRIC DETERMINATION OF CRITICAL MICELLE CONCENTRATION AVOIDING INTERFERENCE FROM DETERGENT CHARGE
FLUORIMETRIC DETERMINATION OF CRITICAL MICELLE CONCENTRATION AVOIDING INTERFERENCE FROM DETERGENT CHARGE
复制标题
DOI：
10.1016/0003-2697(84)90026-5
复制
发表时间：
1984-01-01
影响因子：
2.9
通讯作者：
LONDON, E
中科院分区：
文献类型：
--
作者：
CHATTOPADHYAY, A;LONDON, E
当洗涤剂溶液与中性荧光分子1，6-二苯基-1，3，5-己三烯混合时，如果洗涤剂超过临界胶束浓度（CMC），则观察到荧光的大幅增加。利用该性质测定了阴离子型[十二烷基硫酸钠、胆酸钠]、不带电的[Triton X-100、辛基葡糖苷]、两性离子型[3-[（3-胆酰胺丙基）二甲铵]-1-丙磺酸盐]和阳离子型洗涤剂[溴化环乙基三甲基铵]的CMC。无论洗涤剂的电荷，CMC得到的同意与其他方法得到的值。该方法灵敏、快速，可为洗涤剂CMC的测定提供一种简便、通用的方法。
Upon mixing detergent solutions with the neutral fluorescent molecule 1,6-diphenyl-1,3,5-hexatriene a large increase in fluorescence is observed if detergent exceeds the critical micelle concentration (CMC). This property was used to determine the CMC of anionic [sodium dodecyl sulfate, sodium cholate], uncharged [Triton X-100, octyl glucoside], zwitterionic [3-[(3-cholamidopropyl)dimethylammonio]-1-propanesulfonate] and catonic detergents [cethyltrimethylammonium bromide]. Regardless of detergent charge, the CMC obtained agree with the values obtained by other methods. This fluorescence assay in both sensitive and rapid, and should provide a simple and general method for determination of CMC of any detergent.