Lactobacillus plantarum vaccine vector expressing hemagglutinin provides protection against H9N2 challenge infection
Lactobacillus plantarum vaccine vector expressing hemagglutinin provides protection against H9N2 challenge infection
复制标题
表达血凝素的植物乳杆菌疫苗载体可提供针对 H9N2 攻击感染的保护
DOI：
10.1016/j.virusres.2015.09.005
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发表时间：
2016-01-04
期刊：
影响因子：
5
通讯作者：
Wang, Chun-Feng
中科院分区：
文献类型：
--
作者：
Shi, Shao-Hua;Yang, Wen-Tao;Wang, Chun-Feng
血凝素（HA）已被证明是一种有效的候选疫苗抗原。树突状细胞靶向肽（DCpep）可以增强免疫应答的鲁棒性。本研究的目的是评估当利用植物乳杆菌NC 8（LactobacillusplantarumNC 8，NC 8）在小鼠和鸡模型中呈递HA-DCpep时，DCpep是否能够增强针对H9 N2 AIV的免疫应答。为了实现这一点，采用了在先前研究中构建的表达HA和DCpep的重组NC 8菌株的粘膜疫苗。口服施用的NC 8-pSIP 409-HA-DCpep在小鼠中引起血凝抑制（HI）抗体的高血清滴度，并且还在小鼠和鸡模型中诱导稳健的T细胞免疫应答。口服施用的NC 8-pSIP 409-HA-DCpep在小鼠中引起血凝抑制（HI）抗体的高血清滴度，并且还在小鼠和鸡模型中诱导稳健的T细胞免疫应答。这些结果表明，重组植物乳杆菌NC 8-pSIP 409-HA-DCpep是针对H9 N2 AIV的有效疫苗候选物。(C)2015 Elsevier B. V.版权所有。
Hemagglutinin (HA) has been demonstrated as an effective candidate vaccine antigen against AIVs. Dendritic cell-targeting peptide (DCpep) can enhance the robustness of immune responses. The purpose of this study was to evaluate whether DCpep could enhance the immune response against H9N2 AIV when utilizing Lactobacillus plantarum NC8 (NC8) to present HA-DCpep in mouse and chicken models. To accomplish this, a mucosal vaccine of a recombinant NC8 strain expressing HA and DCpep that was constructed in a previous study was employed. Orally administered NC8-pSIP409-HA-DCpep elicited high serum titers of hemagglutination-inhibition (HI) antibodies in mice and also induced robust T cell immune responses in both mouse and chicken models. Orally administered NC8-pSIP409-HA-DCpep elicited high serum titers of hemagglutination-inhibition (HI) antibodies in mice and also induced robust T cell immune responses in both mouse and chicken models. These results revealed that recombinant L plantarum NC8-pSIP409-HA-DCpep is an effective vaccine candidate against H9N2 AIVs. (C) 2015 Elsevier B.V. All rights reserved.