Formate-Dependent Microbial Conversion of CO2 and the Dominant Pathways of Methanogenesis in Production Water of High-temperature Oil Reservoirs Amended with Bicarbonate.
Formate-Dependent Microbial Conversion of CO2 and the Dominant Pathways of Methanogenesis in Production Water of High-temperature Oil Reservoirs Amended with Bicarbonate.
复制标题
碳酸氢盐修正的高温油藏采出水中二氧化碳的甲酸盐依赖性微生物转化和产甲烷的主要途径
DOI：
10.3389/fmicb.2016.00365
复制
发表时间：
2016
影响因子：
5.2
通讯作者：
Mu BZ
中科院分区：
文献类型：
--
作者：
Yang GC;Zhou L;Mbadinga SM;Liu JF;Yang SZ;Gu JD;Mu BZ
在包括油藏在内的深层地下地层中封存CO2是降低大气中CO2浓度的潜在措施。然而，在二氧化碳捕获和储存（CDCS）设施的CO2的命运和生态影响还不清楚。在当前的研究中，CO2（碳酸氢盐形式; 0 - 90 mM）与10 mM的甲酸盐作为电子供体和碳源的命运进行了研究，与高温生产水在中国的油田。同位素数据表明，碳酸氢盐可以被甲烷菌还原为甲烷，甲烷的主要途径可能是互养甲酸氧化与CO2还原和甲酸甲烷的厌氧条件下。碳酸氢盐的加入引起了微生物群落的变化。碳酸氢盐和甲酸盐的添加与甲烷菌目的减少有关，但在所有处理中甲烷菌目的促进。所有样品中均以嗜热弧菌属为主，高碳酸氢盐处理中产热菌属占优势。结果表明，CDCS中的CO2可以转化为甲烷，微生物转化CO2提高油藏微生物能量采收率的可能性。
CO2 sequestration in deep-subsurface formations including oil reservoirs is a potential measure to reduce the CO2 concentration in the atmosphere. However, the fate of the CO2 and the ecological influences in carbon dioxide capture and storage (CDCS) facilities is not understood clearly. In the current study, the fate of CO2 (in bicarbonate form; 0∼90 mM) with 10 mM of formate as electron donor and carbon source was investigated with high-temperature production water from oilfield in China. The isotope data showed that bicarbonate could be reduced to methane by methanogens and major pathway of methanogenesis could be syntrophic formate oxidation coupled with CO2 reduction and formate methanogenesis under the anaerobic conditions. The bicarbonate addition induced the shift of microbial community. Addition of bicarbonate and formate was associated with a decrease of Methanosarcinales, but promotion of Methanobacteriales in all treatments. Thermodesulfovibrio was the major group in all the samples and Thermacetogenium dominated in the high bicarbonate treatments. The results indicated that CO2 from CDCS could be transformed to methane and the possibility of microbial CO2 conversion for enhanced microbial energy recovery in oil reservoirs.