LIPOPOLYSACCHARIDES OF AEROBACTER AEROGENES STRAINS A3(S1) AND NCTC 243
LIPOPOLYSACCHARIDES OF AEROBACTER AEROGENES STRAINS A3(S1) AND NCTC 243
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DOI：
10.1021/bi00848a032
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发表时间：
1968-01-01
期刊：
影响因子：
2.9
通讯作者：
CONRAD, HE
中科院分区：
文献类型：
--
作者：
KOELTZOW, DE;EPLEY, JD;CONRAD, HE
产气假单胞菌A3(S1)和NCTC 243的脂多糖在温和的水解条件下都能产生一种多糖组分，它是单糖含量和相对分子质量不同的片段的复杂混合物，但葡萄糖和七糖的比例相对恒定。A3(S1)多糖的这一比例约为0.5，NCTC 243的多糖比例约为2。来自这两种脂多糖的多糖组分含有一个支化的半乳聚糖，它具有1个[前进箭头]3个链接的主链和1个[前进箭头]4个链接的分支；这些聚合物似乎只是在它们的分支程度上有结构上的不同。描述了用于定量存在于浓度低至0.1u摩尔/毫升的水解物中的还原糖的同位素方法，以及用于结构未知的毫克量多糖的甲基化分析。
The lipopolysaccharides from A. aerogenes strains A3(S1) and NCTC 243 on mild hydrolysis each yield a polysaccharide fraction which is a complex mixture of fragments varying both in monosaccharide content and molecular weight but having a relatively constant ratio of glucose to heptose. This ratio is approximately 0.5 for the A3(S1) polysaccharide and 2 for that from NCTC 243. The polysaccharide fractions derived from both lipopolysaccharides contain a branched galactan which has 1[forward arrow]3-linked main chains and 1[forward arrow]4-linked branches; these polymers appear to differ structurally only in their degree of branching. Isotopic methods are described for quantitation of reducing saccharides present in hydrolysates at concentrations down to 0.1 u mole/ml and for methylation analysis of milligram amounts of polysaccharides of unknown structure.