Auxin and ETTIN in Arabidopsis gynoecium morphogenesis.
Auxin and ETTIN in Arabidopsis gynoecium morphogenesis.
复制标题
DOI：
--
复制
发表时间：
2000-09
期刊：
影响因子：
4.6
通讯作者：
J. Nemhauser;Lewis J. Feldman;Patricia Zambryski
中科院分区：
文献类型：
--
作者：
J. Nemhauser;Lewis J. Feldman;Patricia Zambryski
植物激素生长素对生长发育有广泛的影响。遗传和生理途径在决定花器官数目和模式方面涉及生长素通量。为了进一步研究生长素在器官发生中的作用，本研究采用了一种新的技术，即瞬时应用生长素运输抑制剂N-1-萘基邻苯二甲酸(NPA)来发育拟南芥的花。在野生型雌蕊群中，NPA对花器官数量和区域规格有显著影响，如区域边界的形态和组织学标志以及组织特异性报告线所定义的那样。NPA对雌蕊群构型的影响模仿了ETTIN的突变表型，ETTIN是生长素反应因子转录因子家族的成员。此外，应用不同浓度的NPA显示弱Ettin等位基因对极性生长素运输中断的敏感性增加。相比之下，骨盆刮片中发现的缺陷可以通过NPA治疗部分恢复。提出了一个模型，提出了在雌蕊群发育过程中生长素的顶端-底端梯度。这个模型提供了一种机制，将ETTIN对生长素反应基因的假定转录调控与雌核发育过程中组织模式的建立或阐述联系起来。
The phytohormone auxin has wide-ranging effects on growth and development. Genetic and physiological approaches implicate auxin flux in determination of floral organ number and patterning. This study uses a novel technique of transiently applying a polar auxin transport inhibitor, N-1-naphthylphthalamic acid (NPA), to developing Arabidopsis flowers to further characterize the role of auxin in organogenesis. NPA has marked effects on floral organ number as well as on regional specification in wild-type gynoecia, as defined by morphological and histological landmarks for regional boundaries, as well as tissue-specific reporter lines. NPA's effects on gynoecium patterning mimic the phenotype of mutations in ETTIN, a member of the auxin response factor family of transcription factors. In addition, application of different concentrations of NPA reveal an increased sensitivity of weak ettin alleles to disruptions in polar auxin transport. In contrast, the defects found in spatula gynoecia are partially rescued by treatment with NPA. A model is proposed suggesting an apical-basal gradient of auxin during gynoecium development. This model provides a mechanism linking ETTIN's putative transcriptional regulation of auxin-responsive genes to the establishment or elaboration of tissue patterning during gynoecial development.