Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis
Distinct requirements for wnt9a and irf6 in extension and integration mechanisms during zebrafish palate morphogenesis
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DOI：
10.1242/dev.080473
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发表时间：
2013-01-01
期刊：
影响因子：
4.6
通讯作者：
Liao, Eric C.
中科院分区：
文献类型：
--
作者：
Dougherty, Max;Kamel, George;Liao, Eric C.
脊椎动物的腭部发育包括颅神经脊的移动、面部隆起的会聚和软骨框架的延伸。腭裂是最常见的结构性先天缺陷，腭突发育失调会导致口裂。对斑马鱼腭部发生的详细分析揭示了腭部形态发生的不同机制：伸展、增殖和整合。我们发现，wnt9a是腭部伸展所必需的，其中软骨细胞形成增殖面，经历形态变化，并插入形成筛板。同时，irf6特别适用于V形接缝的面部隆起的整合。这项工作提出了一个在临床相关背景下的腭部形态发生的机制分析。
Development of the palate in vertebrates involves cranial neural crest migration, convergence of facial prominences and extension of the cartilaginous framework. Dysregulation of palatogenesis results in orofacial clefts, which represent the most common structural birth defects. Detailed analysis of zebrafish palatogenesis revealed distinct mechanisms of palatal morphogenesis: extension, proliferation and integration. We show that wnt9a is required for palatal extension, wherein the chondrocytes form a proliferative front, undergo morphological change and intercalate to form the ethmoid plate. Meanwhile, irf6 is required specifically for integration of facial prominences along a V-shaped seam. This work presents a mechanistic analysis of palate morphogenesis in a clinically relevant context.