The effect of inoculum source and fluid shear force on the development of in vitro oral multispecies biofilms
The effect of inoculum source and fluid shear force on the development of in vitro oral multispecies biofilms
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DOI：
10.1111/jam.13376
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发表时间：
2017-03-01
影响因子：
4
通讯作者：
Rickard, A. H.
中科院分区：
文献类型：
--
作者：
Fernandez, C. E.;Aspiras, M. B.;Rickard, A. H.
目的：唾液先前已被用作体外口腔生物膜研究的接种物。然而，唾液的微生物群落特征与硬组织和软组织相关的口腔生物膜明显不同。在这里，我们调查的生物膜结构和微生物多样性的体外口腔生物膜的变化，从唾液，舌头或菌斑衍生的接种物在不同的唾液剪切力。方法和结果：四种接种物类型（唾液，细菌收获的舌头，牙刷和刮匙收获的斑块）收集和合并。生物膜（n ≥ 15）在三种不同剪切力下流动的无细胞人唾液中生长20小时。使用共聚焦激光扫描显微镜对染色的生物膜进行成像。通过图像分析确定生物量、厚度和粗糙度，并使用Ion Torrent分析细菌群落组成。所有开发的生物膜显示出显着减少观察到的多样性相比，其各自的原始接种物。剪切力改变生物膜结构的唾液和刮匙收集的斑块和社区组成的唾液，舌和刮匙收获plaeconclusion.Conclusions：不同的口内接种体担任前体的体外口腔多微生物生物膜，可以受到shear.Significance和影响的研究：接种体的选择和剪切力是关键因素时，要考虑在体外模型开发多物种生物膜。
Aims: Saliva has been previously used as an inoculum for invitro oral biofilm studies. However, the microbial community profile of saliva is markedly different from hard- and soft-tissue-associated oral biofilms. Here, we investigated the changes in the biofilm architecture and microbial diversity of invitro oral biofilms developed from saliva, tongue or plaque-derived inocula under different salivary shear forces.Methods and Results: Four inoculum types (saliva, bacteria harvested from the tongue, toothbrush and curette-harvested plaque) were collected and pooled. Biofilms (n >= 15) were grown for 20 h in cell-free human saliva flowing at three different shear forces. Stained biofilms were imaged using a confocal laser scanning microscope. Biomass, thickness and roughness were determined by image analysis and bacterial community composition analysed using Ion Torrent. All developed biofilms showed a significant reduction in observed diversity compared with their respective original inoculum. Shear force altered biofilm architecture of saliva and curette-collected plaque and community composition of saliva, tongue and curette-harvested plaque.Conclusions: Different intraoral inocula served as precursors of invitro oral polymicrobial biofilms which can be influenced by shear.Significance and Impact of the Study: Inoculum selection and shear force are key factors to consider when developing multispecies biofilms within invitro models.