Inactivation of viruses in bubbling processes utilized for personal bioaerosol monitoring
Inactivation of viruses in bubbling processes utilized for personal bioaerosol monitoring
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DOI：
10.1128/aem.70.12.6963-6967.2004
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发表时间：
2004-12-01
影响因子：
4.4
通讯作者：
Agranovski, V
中科院分区：
文献类型：
--
作者：
Agranovski, IE;Safatov, AS;Agranovski, V
最近开发了一种新的个人生物气溶胶采样器，并对其进行了评价，用于在受控实验室条件下和实地对空气中的活细菌和真菌进行采样。该装置的工作原理是基于空气通过浸入液体中的多孔介质的通道。当载气通过时，该过程导致在过滤器内形成气泡，从而提供用于气溶胶去除的有效机制。如先前的研究所示，在整个8小时采样期间，采样的细菌和真菌的可培养性保持较高。本研究是评价用于监测空气中活病毒的新采样器的第一步。重点考察连续运行4 h鼓泡过程中病毒灭活率。在这项研究中使用了四种微生物，流感病毒、麻疹病毒、腮腺炎病毒和牛痘病毒。发现使用蒸馏水作为收集流体与相对高的衰减率相关。通过使用Hank溶液和适当的添加剂制备的病毒维持液，实现了显著的改善。与通过无菌水鼓泡相比，在操作的第一个小时后，流感、麻疹和腮腺炎病毒的存活率分别增加了1.4 log、0.83 log和0.82 log。在整个4小时实验中观察到相同的趋势。仅在稳健牛痘病毒中未观察到显著差异。
A new personal bioaerosol sampler has recently been developed and evaluated for sampling of viable airborne bacteria and fungi under controlled laboratory conditions and in the field. The operational principle of the device is based on the passage of air through porous medium immersed in liquid. This process leads to the formation of bubbles within the filter as the carrier gas passes through and thus provides effective mechanisms for aerosol removal. As demonstrated in previous studies, the culturability of sampled bacterium and fungi remained high for the entire 8-h sampling period. The present study is the first step of the evaluation of the new sampler for monitoring of viable airborne viruses. It focuses on the investigation of the inactivation rate of viruses in the bubbling process during 4 h of continuous operation. Four microbes were used in this study, influenza, measles, mumps, and vaccinia viruses. It was found that the use of distilled water as the collection fluid was associated with a relatively high decay rate. A significant improvement was achieved by utilizing virus maintenance fluid prepared by using Hank's solution with appropriate additives. The survival rates of the influenza, measles, and mumps viruses were increased by 1.4 log, 0.83 log, and 0.82 log, respectively, after the first hour of operation compared to bubbling through the sterile water. The same trend was observed throughout the entire 4-h experiment. There was no significant difference observed only for the robust vaccinia virus.