Computational analyses of CO-rebreathing methods for estimating haemoglobin mass in humans.
Computational analyses of CO-rebreathing methods for estimating haemoglobin mass in humans.
复制标题
用于估算人体血红蛋白质量的二氧化碳再呼吸方法的计算分析。
DOI：
10.1113/expphysiol.2011.059436
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发表时间：
2012
影响因子：
2.7
通讯作者：
Bruce,EugeneN
中科院分区：
文献类型：
--
作者：
Chada,KinneraE;Bruce,EugeneN
血红蛋白质量（MHb）的测量用于量化运动训练或适应海拔期间氧输送的变化。血红蛋白对一氧化碳的摄取是测定人类血红蛋白的常用非放射性方法的基础。本研究使用了一个有效的数学模型来模拟CO的吸收在再呼吸协议，并确定来源的错误估计ofMHb。我们先前发表的模型使用在CO再呼吸方案期间从人类受试者的动脉、毛细血管和静脉血液部位实验测量的碳氧血红蛋白水平（%HbCO）进行了验证。然后使用该模型模拟24名已知MHb的人类受试者的各种CO再呼吸方案。使用由模型产生的变量，MHb是基于通常用于计算与肌红蛋白结合的CO体积、呼出的CO体积和再呼吸系统中的CO体积的假设来估计的。结果表明，对肌红蛋白结合CO体积的不准确估计是MHb测定误差的主要来源。此外，由于%HbCO的差异，发现误差的大小取决于血液采样部位。回归方程的开发，以提高估计的CO绑定到肌红蛋白的体积，并提出了一个新的协议，这是不太依赖于现场的血液采样。
Measurement of haemoglobin mass (MHb) is used to quantify alterations in oxygen delivery during exercise training or acclimatization to altitude. Uptake of carbon monoxide by haemoglobin is the basis of the common non‐radioactive methods to determineMHbin humans. This study used a validated mathematical model to simulate CO uptake during rebreathing protocols and to determine sources of errors in estimation ofMHb. Our previously published model was validated using experimentally measured carboxyhaemoglobin levels (%HbCO) from arterial, capillary and venous blood sites of human subjects during CO‐rebreathing protocols. This model was then used to simulate various CO‐rebreathing protocols in 24 human subjects with knownMHb. Using variables generated by the model,MHbwas estimated on the basis of assumptions typically made for calculating the volume of CO bound to myoglobin, the volume of CO exhaled and the volume of CO in the rebreathing system. It was found that inaccurate estimation of the volume of CO bound to myoglobin was the major source of error in determination ofMHb. Additionally, the size of the error was found to depend on the site of blood sampling because of differences in %HbCO. Regression equations were developed to improve the estimation of volume of CO bound to myoglobin, and a new protocol that is less dependent on the site of blood sampling is proposed.