RECOMBINATION IN AIDS VIRUSES
RECOMBINATION IN AIDS VIRUSES
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DOI：
10.1007/bf00163230
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发表时间：
1995-03-01
影响因子：
3.9
通讯作者：
SHARP, PM
中科院分区：
文献类型：
--
作者：
ROBERTSON, DL;HAHN, BH;SHARP, PM
复制有助于人类免疫缺陷病毒（HIV）遗传多样性的产生，但只能发生在同一细胞内复制的病毒之间。由于尚未发现个体同时感染多种不同的HIV-1或HIV-2毒株，因此重组事件被认为仅限于在HIV感染过程中进化的准种的相当密切相关的成员。在这里，我们描述了HIV-1和HIV-2基因组的例子，这些基因组似乎是遗传上完全不同的病毒的杂交体。系统发育分析被用来研究多个HIV毒株之间的进化关系。来自不同基因组区域的进化树与大多数研究的病毒是一致的。然而，HIV-1和HIV-2的一些菌株表现出显着不一致的分支顺序指示在其进化历史的遗传交换。这些假定的重组事件的交叉点进行了映射，通过检查支持替代树拓扑结构的遗传信息位点的分布。最近也描述了在西非绿色猴中鉴定出的重组猿免疫缺陷病毒的类似例子。这些结果表明，高度不同的病毒株的共感染可以发生在HIV感染的人和SIV感染的灵长类动物，并可能导致产生的杂交基因组与显着改变的生物学特性。因此，灵长类慢病毒的未来表征应包括可能的基因组镶嵌仔细的系统发育调查。
Recombination contributes to the generation of genetic diversity in human immunodeficiency viruses (HIV) but can only occur between viruses replicating within the same cell. Since individuals have not been found to be simultaneously coinfected with multiple divergent strains of HIV-1 or HIV-2, recombination events have been thought to be restricted to the rather closely related members of the quasispecies that evolves during the course of HIV infection. Here we describe examples of both HIV-1 and HIV-2 genomes that appear to be hybrids of genetically quite divergent viruses. Phylogenetic analyses were used to examine the evolutionary relationships among multiple HIV strains. Evolutionary trees derived from different genomic regions were consistent with respect to most of the viruses investigated. However, some strains of HIV-1 and HIV-2 exhibited significantly discordant branching orders indicative of genetic exchanges during their evolutionary histories. The crossover points of these putative recombination events were mapped by examining the distribution of phylogenetically informative sites supporting alternative tree topologies. A similar example of a recombinant simian immunodeficiency virus identified in West African green monkeys has also been described recently. These results indicate that coinfection with highly divergent viral strains can occur in HIV-infected humans and SIV-infected primates and could lead to the generation of hybrid genomes with significantly altered biological properties. Thus, future characterization of primate lentiviruses should include careful phylogenetic investigation of possible genomic mosaicism.