Transcriptional regulation of the cinnamyl alcohol dehydrogenase gene from sweet potato in response to plant developmental stage and environmental stress.
Transcriptional regulation of the cinnamyl alcohol dehydrogenase gene from sweet potato in response to plant developmental stage and environmental stress.
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甘薯肉桂醇脱氢酶基因的转录调控响应植物发育阶段和环境胁迫。
DOI：
10.1007/s00299-010-0864-2
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发表时间：
2010-07
影响因子：
6.2
通讯作者：
Huh, Gyung-Hye
中科院分区：
文献类型：
--
作者：
Kim, Young-Hwa;Bae, Jung Myung;Huh, Gyung-Hye
关键词：
肉桂醇脱氢酶（CAD）是木质素生物合成中的一种关键酶。我们已经分离出了一个编码CAD的全长cDNA（IbCAD1），它先前被鉴定为甘薯悬浮细胞的EST文库中最丰富的基因。系统发育分析表明，IbCAD1属于防御相关的CAD家族。在甘薯的根中发现了高水平的IbCAD1 mRNA，但在叶和叶柄中没有。IbCAD1基因转录本受到寒冷、创伤和活性氧物质的高度诱导。对携带IbCAD1启动子 - GUS的转基因烟草植株中IbCAD1基因转录调控的分析显示，IbCAD1启动子在根中的表达很强，但在子叶中几乎检测不到。IbCAD1启动子活性随着根龄的增加而增加，并且在侧根发生部位和根尖观察到强启动子表达。在成熟叶和茎的维管系统木质化组织中观察到弱的GUS表达。IbCAD1启动子活性在生物和非生物胁迫下被强烈诱导，其中最强的诱导因素是创伤，并且也被水杨酸（SA）和茉莉酸（JA）以及脱落酸（ABA）和6 - 苄基氨基嘌呤诱导。综上所述，我们的数据表明IbCAD1可能分别参与JA和SA介导的创伤反应以及ABA介导的寒冷反应。IbCAD1基因可能在生物和非生物胁迫的抗性机制以及组织特异性发育木质化中发挥作用。
Cinnamyl alcohol dehydrogenase (CAD) is a key enzyme in the biosynthesis of lignin. We have isolated full length of a cDNA encoding CAD (IbCAD1) that was previously identified as the most abundant gene in an EST library of sweetpotato suspension cells. Phylogenetic analysis revealed that IbCAD1 belongs to the family of defense-related CADs. High levels of IbCAD1 mRNA were found in the roots of sweetpotato, but not in the leaves and petioles. The IbCAD1 gene transcripts were highly induced by cold, wounding, and reactive oxygen species. Analyses of transcriptional regulation of the IbCAD1 gene in transgenic tobacco plants carrying the IbCAD1 promoter–GUS revealed that IbCAD1 promoter expression was strong in the roots, but barely detectable in the cotyledons. IbCAD1 promoter activity increased with increasing root age, and strong promoter expression was observed in the lateral root emergence sites and in root tips. Weak GUS expression was observed in lignified tissues of vascular system of mature leaves and stems. IbCAD1 promoter activity was strongly induced in response to the biotic and abiotic stresses, with the strongest inducer being wounding, and was also induced by salicylic acid (SA) and jasmonic acid (JA) as well as by abscisic acid (ABA) and 6-benzylaminopurine. Taken together, our data suggest that IbCAD1 can be involved in JA- and SA-mediated wounding response and ABA-mediated cold response, respectively. The IbCAD1 gene may play a role in the resistance mechanism to biotic and abiotic stresses as well as in tissue-specific developmental lignification.
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影响因子：
2.9
作者：
Kim, Young Hwa;Hur, Cheol Goo;Huh, Gyung Hye
通讯作者：
Huh, Gyung Hye
影响因子：
5.1
作者：
KNIGHT, ME;HALPIN, C;SCHUCH, W
通讯作者：
SCHUCH, W
DOI：
10.1016/0167-4838(93)90063-w
发表时间：
1993-09-03
期刊：
BIOCHIMICA ET BIOPHYSICA ACTA
影响因子：
--
作者：
MCKIE, JH;JAOUHARI, R;GORRICHON, L
通讯作者：
GORRICHON, L
影响因子：
11.6
作者：
Abe, H;Urao, T;Yamaguchi-Shinozaki, K
通讯作者：
Yamaguchi-Shinozaki, K
影响因子：
4.3
作者：
GOFFNER, D;JOFFROY, I;BOUDET, AM
通讯作者：
BOUDET, AM