Cyclic AMP-mediated growth arrest is associated with increased expression of human T cell leukemia virus type I structural and transforming genes.
Cyclic AMP-mediated growth arrest is associated with increased expression of human T cell leukemia virus type I structural and transforming genes.
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环 AMP 介导的生长停滞与人 T 细胞白血病病毒 I 型结构和转化基因的表达增加有关。
DOI：
10.1089/aid.1996.12.527
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
Sodroski,JG
中科院分区：
文献类型：
--
作者：
Poteat,HT;Ramstedt,U;Yoon,S;Dardik,M;Terwilliger,E;Sodroski,JG
检测了细胞内环AMP水平增加对人T细胞白血病病毒I型（HTLV-I）前病毒基因表达的影响。诱导感染细胞产生升高水平的环AMP与病毒表面抗原表达、蛋白质合成、p24释放到上清液中和RNA水平的特异性增加相关。观察到的HTLV-I前病毒基因表达模式支持了Tax、雷克斯和环AMP在HTLV-I基因调控中的功能的转染实验结果。证明胸苷掺入，治疗感染的细胞产生环AMP引起可逆的生长停滞。数据表明，HTLV-I RNA和蛋白质合成可以在细胞生长不存在的情况下以升高的水平进行。细胞内cAMP水平的持续增加可能代表了一种在细胞培养物中富集HTLV-I蛋白的方法。
The effect of increased intracellular cyclic AMP levels on gene expression of the human T cell leukemia virus type I (HTLV-I) provirus was examined. Induction of infected cells to produce elevated levels of cyclic AMP was associated with specific increases in viral surface antigen expression, protein synthesis, p24 release into the supernatant, and RNA levels. The patterns of HTLV-I proviral gene expression observed support results from transfection experiments regarding the function of Tax, Rex, and cyclic AMP in HTLV-I gene regulation. As evidenced by thymidine incorporation, treatment of the infected cells to produce cyclic AMP caused reversible growth arrest. The data indicate that HTLV-I RNA and protein synthesis can proceed at an elevated level in the absence of cell growth. Sustained increases in the intracellular level of cyclic AMP may represent a method for enriching cell cultures in HTLV-I proteins.