Ferrite nanospheres-based magnetic solid-phase extraction for determination of domoic acid in seawater samples using high-performance liquid chromatography with tandem mass spectrometry
Ferrite nanospheres-based magnetic solid-phase extraction for determination of domoic acid in seawater samples using high-performance liquid chromatography with tandem mass spectrometry
复制标题
基于铁氧体纳米球的磁性固相萃取，使用高效液相色谱-串联质谱法测定海水样品中的软骨藻酸
DOI：
10.1016/j.chroma.2016.03.055
复制
发表时间：
2016-04-22
影响因子：
4.1
通讯作者：
Zhang, Lan
中科院分区：
文献类型：
--
作者：
Zhang, Wenmin;Lin, Mingxia;Zhang, Lan
建立了一种简便、快速、灵敏的测定海水中软骨藻酸(DA)的方法，该方法基于磁性固相萃取(MSPE)-高效液相色谱-串联质谱仪(LC-MS/MS)。制备了5种铁氧体磁性纳米球(MFe2O4；M=Fe、Co、Ni、Cu和Zn)，并首次用于吸附DA的MSPE和消除盐的干扰。在相同的萃取和洗脱条件下，CuFe2O4磁性纳米球的前处理性能最好，并对其进行了详细的表征。通过对实验条件的进一步优化，方法的线性范围为5~1000pgmL(-1)，检出限为2.5pgmL(-1)，定量下限为5.0pgmL(-1)，S/N=10：1，回收率为86.0~98.1%，重复性较好。
A simple, rapid and sensitive method for determination of trace levels of domoic acid (DA) in seawater was developed, based on a magnetic solid-phase extraction (MSPE) followed by high-performance liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Five kinds of ferrite magnetic nanospheres (MFe2O4; M = Fe, Co, Ni, Cu and Zn) were prepared and first used as sorbents for MSPE of DA and removal of salt interference. Under the same extraction and elution conditions, CuFe2O4 magnetic nanospheres provided the best pretreatment performance, which were then characterized in detail. After further optimization of conditions, the developed method showed good linearity (r(2) = 0.9991) with the range of 5-1000 pg mL(-1), low limit of detection (2.5 pg mL(-1); SiN =3:1), low limit of quantification (5.0 pg mL(-1); S/N = 10:1), and good recoveries (86.0-98.1%) with acceptable repeatability (RSD