PARTICIPATION OF SOLUBLE PHOSPHATIDYLSERINE SYNTHETASE IN PHOSPHATIDYLETHANOLAMINE BIOSYNTHESIS IN ESCHERICHIA-COLI MEMBRANE
PARTICIPATION OF SOLUBLE PHOSPHATIDYLSERINE SYNTHETASE IN PHOSPHATIDYLETHANOLAMINE BIOSYNTHESIS IN ESCHERICHIA-COLI MEMBRANE
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DOI：
10.1111/j.1432-1033.1974.tb03362.x
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发表时间：
1974-01-01
期刊：
影响因子：
--
通讯作者：
KITO, M
中科院分区：
文献类型：
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作者：
ISHINAGA, M;KITO, M
磷脂酰丝氨酸合成酶在大肠杆菌中以可溶性和核糖体结合的形式存在。用DEAE-Sephadex柱层析对大肠杆菌B进行了部分纯化。采用差速离心法获得核糖体酶。两种类型的酶具有相同的Km(150μM)的1-丝氨酸。通过膜中的CDP-二甘油酯从磷脂酸生物合成磷脂酰乙醇胺所需的可溶酶。核糖体酶对磷脂酰乙醇胺的形成不如可溶性酶有效。蔗糖密度梯度离心法证明可溶性酶与膜结合松散。
Phosphatidylserine synthetase occurs in a soluble and a ribosome‐bound form inEscherichia coli.The solble enzyme ofE. coliB was partially purified by DEAE‐Sephadex chromatography. The ribosomal enzyme was obtained by differential centrifugation. Two types of the enzyme had the sameKm(150 μM) forl‐serine. The soluble enzyme was required for the biosynthesis of phosphatidylethanolamine from phosphatidic acidviaCDP‐diglyceride in the membrane. The ribosomal enzyme was less effective to the phosphatidylethanolamine formation than the soluble enzyme. By the sucrose‐density‐gradient centrifugation method the soluble enzyme was proved to be loosely associated with the membrane.