Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression
Streptavidin suppresses T cell activation and inhibits IL-2 production and CD25 expression
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DOI：
10.1016/j.cyto.2012.02.007
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发表时间：
2012-06-01
期刊：
影响因子：
3.8
通讯作者：
Chu, Cong-Qiu
中科院分区：
文献类型：
--
作者：
Yomogida, Kentaro;Chou, Yuan;Chu, Cong-Qiu
链霉亲和素因其与生物素的高亲和性和特异性结合而被广泛用作生物学研究中的检测工具。生物素-链霉亲和素系统在临床医学中也被探索用于感染和肿瘤的检测。在这里，我们展示了链霉亲和素对T细胞活化和增殖的免疫抑制特性。在CD 3和CD 28刺激下，CD 4（+）T细胞产生白细胞介素2（IL-2）并表达IL-2受体α链（CD 25）。在T细胞培养物中添加链霉亲和素抑制IL-2合成和CD 25表达，而无细胞毒性。过量的生物素可逆转链霉亲和素的免疫抑制作用。与单链抗CD 7可变片段（scFvCD 7）缀合，链霉亲和素被直接递送至T细胞，并且对T细胞活化显示出显著更深刻的抑制作用。这些结果表明，链霉亲和素可能被用作一种新的免疫调节剂。爱思唯尔有限公司出版
Streptavidin is widely used as a detection tool in biology research because of its high affinity and specificity binding to biotin. Biotin-streptavidin system has also been explored for detection of infection and tumor in clinical medicine. Here, we show immunosuppressive property of streptavidin on T cell activation and proliferation. Upon CD3 and CD28 stimulation, CD4(+) T cells produce interleukin 2 (IL-2) and express IL-2 receptor alpha chain (CD25). Addition of streptavidin in T cell culture suppressed IL-2 synthesis and CD25 expression with no cytotoxicity. The immunosuppressive effect of streptavidin was reversed by excessive biotin. Conjugated to a single chain anti-CD7 variable fragment (scFvCD7), streptavidin was directly delivered to T cells and showed substantially more profound suppressive effect on T cell activation. These results suggest that streptavidin could potentially be used as a novel immunomodulator. Published by Elsevier Ltd.