A fifteen-amino-acid TVB peptide serves as a minimal soluble receptor for subgroup B avian leukosis and sarcoma viruses
A fifteen-amino-acid TVB peptide serves as a minimal soluble receptor for subgroup B avian leukosis and sarcoma viruses
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DOI：
10.1128/jvi.76.11.5404-5410.2002
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发表时间：
2002-06-01
影响因子：
5.4
通讯作者：
Young, JAT
中科院分区：
文献类型：
--
作者：
Knauss, DJ;Young, JAT
亚型B、D和E禽肉瘤和白血病病毒（ASLV）的TVB受体是与TRAIL受体最密切相关的肿瘤坏死因子受体相关蛋白。在这里，我们表明，主要的亚组B病毒相互作用决定簇的TVB包含在一个线性的15个氨基酸的肽来自N-末端区域的受体。此外，该肽不仅足以结合ASLV-B，而且还足以激活病毒进入缺乏同源病毒受体的哺乳动物细胞。在巴弗洛霉素A1的存在下，肽依赖性病毒进入被阻断，表明病毒体可以被运输到酸性内体融合区室，而不需要病毒受体与细胞膜的物理连接。
The TVB receptor for subgroup B, D, and E avian sarcoma and leukosis viruses (ASLVs) is a tumor necrosis factor receptor-related protein that is most closely related to the TRAIL receptors. Here we show that the major subgroup B viral interaction determinants of TVB are contained within a linear 15-amino-acid peptide derived from the N-terminal region of the receptor. Moreover, this peptide was sufficient not only for binding to ASLV-B but also for activating viral entry into mammalian cells that lacked the cognate viral receptor. Peptide-dependent viral entry was blocked in the presence of bafilomycin A1, indicating that virions can be trafficked to an acidic endosomal fusion compartment without the need for physical attachment of the viral receptor to a cellular membrane.