Selection of High-Quality Spermatozoa May Be Promoted by Activated Vitamin D in the Woman
Selection of High-Quality Spermatozoa May Be Promoted by Activated Vitamin D in the Woman
复制标题
DOI：
10.1210/jc.2016-3008
复制
发表时间：
2017-03-01
影响因子：
5.8
通讯作者：
Jensen, Martin Blomberg
中科院分区：
文献类型：
--
作者：
Hansen, Lasse Bollehuus;Rehfeld, Anders;Jensen, Martin Blomberg
内容：维生素D受体（VDR）和参与激活的酶维生素D的CYP 2 R1、CYP 27 B1和CYP 24 A1在卵巢、睾丸和精子中表达。测定正常和不育男性精子对1，25-二羟维生素D-3 [1，25（OH）（2）D-3]的反应性，并确定暴露部位和暴露方式1，25（OH）（2）D-3影响精子功能。设计：分析正常和不育男性表达VDR、CYP 2 R1、CYP 27 B1和CYP 24 A1的精子。25-在血清、精液、宫颈分泌物和卵巢卵泡液中测定羟维生素D（25-OHD）、24，25-二羟维生素D [24，25（OH）（2）D-3]和1，25（OH）（2）D-3。1，25（OH）2D 3在人类精子上进行了测试。地点：三级生育中心。参与者：对230名不育男性和114名健康男性的精子蛋白表达和精液质量进行了评估。在245名男性和13名女性的体液中检测了维生素D代谢产物，同时74个卵母细胞和17个精液供体用于精子功能测试。精子中VDR和CYP 24 A1的表达，25-OHD、24，25（OH）（2）D-3和1，25（OH）（2）D-3的液体浓度，以及1，25（OH）（2）D-3对细胞内钙离子浓度（[Ca 2 +]（i））和精子-卵母细胞结合的影响。结果：VDR和CYP 24 A1在正常男性精子中的表达率是不育男性精子的2倍以上（P，0.01）。25-OHD、24，25（OH）（2）D和1，25（OH）（2）D-3在精液中未检测到，但在卵巢卵泡液中较高。结论：VDR和CYP 24 A1主要存在于高质量精子中，提示女性生殖道中1，25（OH）（2）D-3可促进精子对最佳配子的选择。
Context: The vitamin D receptor (VDR) and enzymes involved in activation (CYP2R1, CYP27B1) and inactivation (CYP24A1) of vitamin D are expressed in ovary, testes, and spermatozoa.Objective: Determine responsiveness to 1,25-dihydroxyvitamin D-3 [1,25(OH)(2)D-3] in spermatozoa from normal and infertile men, and identify the site of exposure and how 1,25(OH)(2)D-3 influences sperm function.Design: Spermatozoa expressing VDR, CYP2R1, CYP27B1, and CYP24A1 were analyzed in normal and infertile men. 25-Hydroxyvitamin D (25-OHD), 24,25-dihydroxyvitamin D [24,25(OH)(2)D-3], and 1,25(OH)(2)D-3 were measured in serum, seminal fluid, cervical secretions, and ovarian follicular fluid. 1,25(OH) 2D3 was tested on human spermatozoa.Setting: Tertiary center for fertility.Participants: Protein expression in spermatozoa and semen quality were assessed in 230 infertile and 114 healthy men. Vitamin D metabolites were measured in fluids from 245 men and 13 women, while 74 oocytes and 17 semen donors were used for sperm-function tests.Main Outcome Measures: VDR and CYP24A1 expressions in spermatozoa, fluid concentrations of 25-OHD, 24,25(OH)(2)D-3, and 1,25(OH)(2)D-3, and 1,25(OH)(2)D-3-induced effects on intracellular calcium concentration ([Ca2+](i)) and sperm-oocyte binding in vitro. Results: VDR and CYP24A1 were expressed in a >2-fold higher fraction of spermatozoa from normal than infertile men (P, 0.01). Concentrations of 25-OHD, 24,25(OH)(2)D, and 1,25(OH)(2)D-3 were undetectable in seminal fluid but high in ovarian follicular fluid. Follicular concentrations of 1,25(OH)(2)D-3 induced a modest increase in [Ca2+] i and sperm-oocyte binding in vitro (P,0.05).Conclusion: Presence of VDR and CYP24A1 mainly in spermatozoa of higher quality supports that 1,25(OH)(2)D-3 available in the female reproductive tract may promote selection of the best gametes for fertilization.