Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure.
Yeast osmosensor Sln1 and plant cytokinin receptor Cre1 respond to changes in turgor pressure.
复制标题
酵母OSMOSENSOR SLN1和植物细胞分裂素受体CRE1响应着爆炸压力的变化。
DOI：
10.1083/jcb.200301099
复制
发表时间：
2003-06-23
影响因子：
7.8
通讯作者：
Saito, Haruo
中科院分区：
文献类型：
--
作者：
Reiser, VladimIr;Raitt, Desmond C;Saito, Haruo
很少有人知道细胞的渗透压传感器如何监测环境中的渗透压变化。在这里，我们报告说，在酵母中，Sln1 β传感器组氨酸激酶监测膨压的变化。由高渗应激、制霉菌素或去除细胞壁引起的膨压降低特异性地通过SLN 1分支而不是通过高渗透压甘油途径的SHO1分支激活MAPK Hog1。Sln1的周质区的完整性对其传感器功能是必不可少的。我们发现，活性的植物组氨酸激酶细胞分裂素反应1（Cre1）也调节膨压的变化，在相同的方式，Sln1，在细胞分裂素的存在下。我们建议，Sln1和Cre1是膨压传感器，并且类似的膨压传感机制可能调节酵母和植物的高渗胁迫反应。
Very little is known about how cellular osmosensors monitor changes in osmolarity of the environment. Here, we report that in yeast, Sln1 osmosensor histidine kinase monitors changes in turgor pressures. Reductions in turgor caused by either hyperosmotic stress, nystatin, or removal of cell wall activate MAPK Hog1 specifically through the SLN1 branch, but not through the SHO1 branch of the high osmolarity glycerol pathway. The integrity of the periplasmic region of Sln1 was essential for its sensor function. We found that activity of the plant histidine kinase cytokinin response 1 (Cre1) is also regulated by changes in turgor pressure, in a manner identical to that of Sln1, in the presence of cytokinin. We propose that Sln1 and Cre1 are turgor sensors, and that similar turgor-sensing mechanisms might regulate hyperosmotic stress responses both in yeast and plants.