Insight into the metabolic mechanism of scoparone on biomarkers for inhibiting Yanghuang syndrome.
Insight into the metabolic mechanism of scoparone on biomarkers for inhibiting Yanghuang syndrome.
复制标题
深入探讨太古香酮抑制阳黄证生物标志物的代谢机制。
DOI：
10.1038/srep37519
复制
发表时间：
2016-11-21
影响因子：
4.6
通讯作者：
Wang X
中科院分区：
文献类型：
--
作者：
Fang H;Zhang A;Yu J;Wang L;Liu C;Zhou X;Sun H;Song Q;Wang X
Scoparone（6,7-二甲氧基香豆素）是银陈浩（Artemisia capillaris Thunb.）的代表成分，银陈浩是一种著名的中草药，对各种肝脏疾病都有良好的疗效，特别是治疗阳黄证（YHS）。scoparone作用于YHS的确切分子机制尚未完全阐明。本研究的目的是确定天scoparone的作用机制，并评价其对代谢物水平的影响。研究了天scopone药理作用相关代谢物的差异表达及其对该疾病的保护作用。通过多变量统计分析，使用精确的MS/MS鉴定出33个生物标志物，这些生物标志物在与肝脏疾病相关的关键代谢途径的调节中发挥重要作用。此外，YHS模型组的病理结果也显示出一致的变化，经天scoparone治疗后，代谢谱和组织病理学与正常水平相似，表明在观察期内疗效良好。本研究表明，代谢组学平台为理解天scopone等天然药物的作用机制提供了新的视角。
Scoparone (6,7-dimethoxycoumarin) is the representative ingredient of Yinchenhao (Artemisia capillaris Thunb.) which is a famous Chinese medicinal herb and shows favorable efficacy for all kinds of liver disease, specifically for the treatment of Yanghuang syndrome (YHS). The precise molecular mechanism concerning the action of scoparone on YHS is yet to be fully elucidated. The aim of the present study was to determine the mechanism of scoparone and evaluate its efficacy on metabolite levels. The differential expression of metabolites responsible for the pharmacological effects of scoparone was characterized and the protection effect of scoparone against this disease. Using multivariate statistical analysis, 33 biomarkers were identified using precise MS/MS and play an important role in the regulation of key metabolic pathways associated with liver disease. In addition, pathological results also showed consistent changes in the YHS model group and after treatment with scoparone, both the metabolic profile and histopathology resembled that of normal level, which suggesting favorable efficacy over the observed time period. The present work indicated that a metabolomics platform provided a new insight into understanding the mechanisms of action of natural medicines such as scoparone.