High-rate cotreatment of purified terephthalate and dimethyl terephthalate manufacturing wastewater by a mesophilic upflow anaerobic sludge blanket reactor and the microbial ecology relevant to aromatic compound degradation
High-rate cotreatment of purified terephthalate and dimethyl terephthalate manufacturing wastewater by a mesophilic upflow anaerobic sludge blanket reactor and the microbial ecology relevant to aromatic compound degradation
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DOI：
10.1016/j.watres.2022.118581
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发表时间：
2022-05-15
期刊：
影响因子：
12.8
通讯作者：
Yamada, Masayoshi
中科院分区：
文献类型：
--
作者：
Kuroda, Kyohei;Narihiro, Takashi;Yamada, Masayoshi
聚对苯二甲酸乙二醇酯（PET）在世界范围内生产，主要用作塑料饮料瓶的材料。PET是由纯对苯二甲酸酯（PTA）或对苯二甲酸二甲酯（DMT）与乙二醇聚合而成。在PTA和DMT的合成生产过程中，会产生高有机负荷的废水，通常通过厌氧废水处理技术单独处理。鉴于对PET的高需求，制造工厂正在全球范围内扩张，这将导致需要处理的PTA和DMT废水量增加。在有效处理方面，PTA和DMT废水的共处理具有几个优点，包括较低的面积和能量要求。在这项研究中，我们研究了上流式厌氧污泥床（UASB）反应器在共处理PTA和DMT废水与高有机负荷的性能，评估其去除特性后，518天的连续运行。此外，我们还对UASB颗粒污泥进行了微生物组学分析，以揭示反应器内微生物的相互作用和代谢功能。通过连续运行，我们实现了6.6千克米（-3）天（-1）的有机去除率。此外，我们证实了PTA和DMT生产过程中的复杂废水中的芳香族化合物是可生物降解的，其顺序为：苯甲酸酯>邻苯二甲酸酯>对苯二甲酸酯>邻苯二甲酸酯>对甲基苯甲酸。基于16 S rRNA基因的网络分析表明，厌氧微古菌门Woesearchaeales与甲烷调节菌属Methanoregula、甲烷鞭毛虫属Methanofastidiosum和甲烷八叠球菌属Methanosarcina均呈正相关，表明它们与颗粒污泥中的产甲烷菌存在共生关系。鸟枪宏基因组分析表明，对苯二甲酸酯，对苯二甲酸酯/邻苯二甲酸酯，苯甲酸酯降解的不同成员的Pelotomaculaceae和Syntrophorhabdaceae。根据基因组信息，我们提出了两个新的邻苯二甲酸酯降解的可能途径，由互养杆菌科生物。
Polyethylene terephthalate (PET) is produced worldwide, mainly as material for plastic drink bottles. PET is produced by polymerization of purified terephthalate (PTA) or dimethyl terephthalate (DMT) with ethylene glycol. During the synthetic manufacturing processes of PTA and DMT, high organic loading wastewater is produced, which is typically treated separately by anaerobic wastewater treatment technologies. Given the high demand for PET, manufacturing plants are expanding globally, which will result in an increase in the amounts of PTA and DMT wastewater in need of treatment. In terms of effective treatment, the cotreatment of PTA and DMT wastewater has several advantages, including lower area and energy requirements. In this study, we examined the performance of an upflow anaerobic sludge blanket (UASB) reactor in cotreating PTA and DMT wastewater with high organic loading, evaluating its removal characteristics after 518 days of continuous operation. In addition, we performed a microbiome analysis of the UASB granular sludge to uncover the microbial interactions and metabolic functions within the reactor. By continuous operation, we achieved an organic removal rate of 6.6 kg m(-3) day(-1). In addition, we confirmed that aromatic compounds in the complex wastewater from the PTA and DMT manufacturing processes are biodegradable in the following order: benzoate > orthophthalate > terephthalate > isophthalate > p-toluic acid. 16S rRNA gene based network analysis shows that anaerobic Woesearchaeales belonging to phylum Nanoarchaeota has a positive correlation with Methanoregula, Candidatus Methanofastidiosum, and Methanosarcina, suggesting a symbiotic relationship with methanogens in granular sludge. Shotgun metagenomic analysis revealed that terephthalate, isophthalate/orthophthalate, and benzoate were degraded by different members of Pelotomaculaceae and Syntrophorhabdaceae. According to the genomic information, we propose two new possible routes for orthophthalate degradation by the Syntrophorhabdaceae organism.