Progestin-induced down regulation of nuclear estrogen receptor in uterine decidual cells: analysis of receptor synthesis and turnover by the density-shift method.
Progestin-induced down regulation of nuclear estrogen receptor in uterine decidual cells: analysis of receptor synthesis and turnover by the density-shift method.
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DOI：
10.1016/0006-291x(86)90947-2
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发表时间：
1986-02
影响因子：
3.1
通讯作者：
A. Takeda;W. W. Leavitt-W.
中科院分区：
文献类型：
--
作者：
A. Takeda;W. W. Leavitt-W.
本文用密度位移法研究了人工合成的孕激素R5020（17，21-二甲基-19-去甲-4，9-孕二烯-3，20-二酮）对金黄地鼠蜕膜细胞核雌激素受体更新和合成的影响。用密集的[2 H，13 C，15 N]氨基酸标记新合成的受体，并通过密度梯度离心将其与预先存在的受体分离。孕激素在处理3 h内增加受体更新，并在6 h和9 h阻断雌二醇诱导的受体合成。因此，槲皮素通过增加受体周转和抑制雌激素诱导的受体补充来下调雌激素受体。
The density-shift method was used to study the effect of the synthetic progestin, R5020, (17,21-dimethyl-19-nor-4,9-pregnadiene-3,20-dione) on the turnover and synthesis of nuclear estrogen receptor in hamster decidual cells. Newly-synthesized receptor was labeled with dense [2H,13C,15N] amino acids and separated from pre-existing receptor by density-gradient centrifugation. Progestin increased receptor turnover within 3 h of treatment and blocked estradiol-induced receptor synthesis at 6 h and 9 h. Thus, progestin down regulates estrogen receptor by increasing receptor turnover and inhibiting estrogen-induced receptor replenishment.