MONOCLONAL-ANTIBODIES REACTIVE WITH THE BREAST CARCINOMA-ASSOCIATED MUCIN CORE PROTEIN REPEAT SEQUENCE PEPTIDE ALSO RECOGNIZE THE OVARIAN CARCINOMA-ASSOCIATED SEBACEOUS GLAND ANTIGEN
MONOCLONAL-ANTIBODIES REACTIVE WITH THE BREAST CARCINOMA-ASSOCIATED MUCIN CORE PROTEIN REPEAT SEQUENCE PEPTIDE ALSO RECOGNIZE THE OVARIAN CARCINOMA-ASSOCIATED SEBACEOUS GLAND ANTIGEN
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DOI：
10.1159/000217661
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发表时间：
1990-09-01
期刊：
影响因子：
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通讯作者：
MCKENZIE, IFC
中科院分区：
文献类型：
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作者：
LAYTON, GT;DEVINE, PL;MCKENZIE, IFC
测试了单克隆抗体 (Mab) OM-1（定义卵巢癌相关皮脂腺抗血清 (SGA)）和 Mab F36/22（定义导管癌抗原 (DCA)）针对合成肽的反应性，该合成肽代表人多态性上皮粘蛋白核心蛋白的重复 20 个氨基酸序列加上相邻序列 (p1-24) 的 4 个氨基酸。通过直接斑点印迹测定和ELISA，OM-1 与肽强烈结合，并且通过抑制测定显示OM-1 的最小表位是APDTRP(A)。 F36/22在相同条件下与肽反应较弱，其对溶液中肽的亲和力相对很低。 Mab OC125 定义了卵巢癌相关抗原 CA125，不与 p1-24 肽发生反应。其他五种抗粘蛋白单克隆抗体（HMFG1、HMFG2、BC1、BC2、BC3）先前显示可与 p1-24 肽结合，通过直接结合以及在以 OM-1 作为捕获单克隆抗体的夹心测定中与 SGA 发生强烈反应。 F36/22 在相同条件下呈弱阳性，表明肽和 SGA 都不表达 F36/22 结合的最佳表位。这些结果表明SGA和可能的DCA具有乳腺癌相关人多态性上皮粘蛋白的重复序列核心蛋白。
The monoclonal antibody (Mab) OM-1, which defines the ovarian carcinoma-associated sebaceous gland antisera (SGA), and Mab F36/22, which defines the ductal carcinoma antigen (DCA), were tested for reactivity against a synthetic peptide representing the repeat twenty amino acid sequence of the human polymorphic epithelial mucin core protein plus four amino acids of the adjacent sequence (p1-24). OM-1 bound strongly to the peptide by direct dot blot assay and ELISA and the minimum epitope for OM-1 was shown to be APDTRP(A) by inhibition assay. F36/22 reacted weakly with the peptide under the same conditions and its affinity for peptide in solution was relatively very low. Mab OC125, which defines the ovarian cancer-associated antigen CA125, did not react with the p1-24 peptide. Five other anti-mucin Mabs (HMFG1, HMFG2, BC1, BC2, BC3), previously shown to bind to the p1-24 peptide, reacted strongly with SGA by direct binding and in a sandwich assay with OM-1 as the capture Mab. F36/22 was weakly positive under the same conditions suggesting that both peptide and SGA do not express the optimal epitope for F36/22 binding. These results indicate that SGA and possibly DCA have the repeat sequence core protein of the breast carcinoma-associated human polymorphic epithelial mucin.