EFFECTS OF HUMAN RECOMBINANT INTERLEUKIN-1 ON ELECTRICAL-PROPERTIES OF GUINEA-PIG VENTRICULAR CELLS
EFFECTS OF HUMAN RECOMBINANT INTERLEUKIN-1 ON ELECTRICAL-PROPERTIES OF GUINEA-PIG VENTRICULAR CELLS
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DOI：
10.1093/cvr/27.3.525
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发表时间：
1993-03-01
影响因子：
10.8
通讯作者：
ROZANSKI, GJ
中科院分区：
文献类型：
--
作者：
LI, YH;ROZANSKI, GJ
目的：为了确定细胞因子是否改变心脏细胞的电特性，在豚鼠心室的离体组织和分离的肌细胞中检查了人重组白细胞介素-1 β（IL-1）的作用。研究方法：在第一系列的实验中，记录跨膜电位与1 ng.ml-1 IL-1在存在和不存在花生四烯酸代谢阻滞剂的情况下灌流离体乳头肌。其次，为了研究对IL-1的反应的离子机制，从胶原酶灌注的心脏中分离心室肌细胞，并在设计用于分离L型Ca 2+电流（I（Ca））的条件下使用膜片钳技术的全细胞构型进行研究。结果如下：在离体乳头肌中，IL-1显著延长动作电位时程（在90%复极化时测量）24.2（SEM 2.2）ms和有效不应期22.9（2.3）ms（均为p
Objective: To determine whether cytokines alter the electrical properties of heart cells, the effects of human recombinant interleukin-1beta (IL-1) were examined in excised tissues and dissociated myocytes from guinea pig ventricles. Methods: In a first series of experiments, transmembrane potentials were recorded from isolated papillary muscles superfused with 1 ng.ml-1 IL-1 in the absence and presence of blockers of arachidonic acid metabolism. Secondly, to examine the ionic mechanisms underlying the response to IL-1, ventricular myocytes were dissociated from collagenase perfused hearts and studied using the whole cell configuration of the patch clamp technique under conditions designed to isolate the L-type Ca2+ current (I(Ca)). Results: In excised papillary muscles, IL-1 significantly prolonged action potential duration (measured at 90% repolarisation) by 24.2(SEM 2.2) ms and effective refractory period by 22.9(2.3) ms (both p