NEW SENSITIVE COLORIMETRIC ASSAY FOR PEROXIDASE USING 3,3'-DIAMINOBENZIDINE AS HYDROGEN DONOR
NEW SENSITIVE COLORIMETRIC ASSAY FOR PEROXIDASE USING 3,3'-DIAMINOBENZIDINE AS HYDROGEN DONOR
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DOI：
10.1016/0003-2697(73)90144-9
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发表时间：
1973-01-01
影响因子：
2.9
通讯作者：
FAHIMI, HD
中科院分区：
文献类型：
--
作者：
HERZOG, V;FAHIMI, HD
本文介绍了一种以3，3 ′-二氨基联苯胺四盐酸盐为氢供体的过氧化物酶活力测定新方法。DAB在常规测定条件下稳定，其自动氧化速率可忽略不计。在最佳条件下，过氧化物酶的浓度与3，3 ′-二氨基联苯胺盐酸盐的氧化速率（Δ A405 nm/min）呈线性关系。使用辣根过氧化物酶，DAB法似乎比邻联茴香胺和愈创木酚测定过氧化物酶更敏感。该方法也可用于测量组织组分中的过氧化物酶活性。
A new simple colorimetric assay for measurement of peroxidase activity using 3,3′-diaminobenzidine tetrahydrochloride as hydrogen donor is described. The DAB is stable under the usual assay conditions, and its rate of auto-oxidation is negligible. Under optimal conditions, a linear relationship is found between peroxidase concentration and the rate of oxidation of 3,3′-diaminobenzidine tetrahydrochloride (ΔA405nm/min). Using horseradish peroxidase, the DAB method appears more sensitive than the o-dianisidine and the guaiacol assays for peroxidase. This method can also be used for measurement of peroxidase activity in tissue fractions.