Dual Effect of Amino Modified Polystyrene Nanoparticles on Amyloid β Protein Fibrillation
Dual Effect of Amino Modified Polystyrene Nanoparticles on Amyloid β Protein Fibrillation
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DOI：
10.1021/cn900027u
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发表时间：
2010-04-01
影响因子：
5
通讯作者：
Linse, Sara
中科院分区：
文献类型：
--
作者：
Cabaleiro-Lago, Celia;Quinlan-Pluck, Fiona;Linse, Sara
在不同尺寸的阳离子聚苯乙烯纳米颗粒存在的情况下，分析了淀粉样β肽的原纤维化动力学。结果强调了肽和颗粒浓度之间的比率的重要性。根据具体的比例，动力学效应各不相同，从通过减少溶液中低颗粒表面积的滞后期来加速原纤化过程到在高颗粒表面积处抑制原纤化过程。如果我们假设两种不同途径之间的平衡，则可以解释动力学行为：溶液中游离单体的第一次原纤化和第二次在颗粒表面促进的成核和原纤化。纤维颤动的总体速率将取决于这两种途径之间的相互作用，并且一种机制相对于另一种机制的优势将由相对平衡和速率常数决定。
The fibrillation kinetics of the amyloid beta peptide is analyzed in presence of cationic polystyrene nanoparticles of different size. The results highlight the importance of the ratio between the peptide and particle concentration. Depending on the specific ratio, the kinetic effects vary from acceleration of the fibrillation process by reducing the lag phase at low particle surface area in solution to inhibition of the fibrillation process at high particle surface area. The kinetic behavior can be explained if we assume a balance between two different pathways: first fibrillation of free monomer in solution and second nucleation and fibrillation promoted at the particle surface. The overall rate of fibrillation will depend on the interplay between these two pathways, and the predominance of one mechanism over the other will be determined by the relative equilibrium and rate constants.