Molecular characterization of phycobilisome regulatory mutants of Fremyella diplosiphon.
Molecular characterization of phycobilisome regulatory mutants of Fremyella diplosiphon.
复制标题
双管芙蕾菌藻胆体调节突变体的分子特征。
DOI：
10.1128/jb.171.2.901-908.1989
复制
发表时间：
1989
影响因子：
3.2
通讯作者：
Grossman,AR
中科院分区：
文献类型：
--
作者：
Bruns,BU;Briggs,WR;Grossman,AR
在电穿孔实验过程中，在双倍管弗拉米尔菌中产生了三类色素突变体。红色突变体类在红光和绿色光中都具有高水平的藻红蛋白，并且在红光中没有诱导型藻蓝蛋白。因此，无论光照条件如何，该突变体的行为就好像它总是处于绿色光中。蓝色突变体表现出正常的藻红蛋白光调节，而诱导藻蓝蛋白是目前在红光和绿光生长的细胞在高水平。此外，与我们的野生型菌株相比，别藻蓝蛋白的绝对量增加了三倍。绿色突变体失去了在绿色光下积累藻红蛋白的能力，但表现出正常的藻蓝蛋白光调节。在这些突变体中的转录丰度的分析表明，不同的藻胆体组分的水平的变化与这些组件的mRNA编码的水平的变化相关。这些突变体的表征支持假设先前讨论的分子机制参与调控的藻胆蛋白基因组在色适应。
Three classes of pigment mutants were generated in Fremyella diplosiphon in the course of electroporation experiments. The red mutant class had high levels of phycoerythrin in both red and green light and no inducible phycocyanin in red light. Thus, this mutant behaved as if it were always in green light, regardless of light conditions. Blue mutants exhibited normal phycoerythrin photoregulation, whereas the inducible phycocyanin was present at high levels in both red- and green-light-grown cells. Furthermore, the absolute amount of allophycocyanin was increased threefold in comparison with our wild-type strain. Green mutants lost the capacity to accumulate phycoerythrin in green light but showed normal photoregulation of phycocyanin. Analyses of transcript abundance in these mutants demonstrated that changes in the levels of the different phycobilisome components correlated with changes in the levels of mRNAs encoding those components. The characterization of these mutants supports hypotheses previously discussed concerning molecular mechanisms involved in the regulation of the phycobiliprotein gene sets during chromatic adaptation.