Overexpression of interferon-activated gene 202 (Ifi202) correlates with the progression of autoimmune glomerulonephritis associated with the MRL chromosome 1
Overexpression of interferon-activated gene 202 (Ifi202) correlates with the progression of autoimmune glomerulonephritis associated with the MRL chromosome 1
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DOI：
10.1177/0961203310362534
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发表时间：
2010-07-01
期刊：
影响因子：
2.6
通讯作者：
Kon, Y.
中科院分区：
文献类型：
--
作者：
Ichii, O.;Kamikawa, A.;Kon, Y.
B6.携带狼疮易感MRL 1号染色体端粒区的MRLc 1（82-100）同源小鼠发生自身免疫性肾小球肾炎（GN）。GN易感基因B6。MRLc 1（82-100）包含干扰素激活基因200（Ifi 200）家族，其由Ifi 202、203、204和205组成。最近，Ifi 202被建议作为小鼠狼疮的候选基因。在这项研究中，我们评估了Ifi 200家族与几种疾病模型中的GN之间的关联。我们比较了C57 BL/6和B6之间24个器官中Ifi 200家族成员的表达。MRLc1（82-100）。Ifi 200家族成员的表达在菌株之间存在差异，并且最显著的差异出现在Ifi 202的表达中。简言之，在血液、免疫器官、肺和睾丸中，B6的mRNA表达较高。MRLc 1（82-100）小鼠。在肾脏和免疫器官中，只有Ifi 202的表达随着B6中GN的发展而增加。MRLc 1（82-100），甚至在发病前就观察到与C57 BL/6的显著差异。在BXSB、NZB/WF 1和MRL/lpr的肾脏中，Ifi 202的表达在疾病的早期和晚期也显著高。此外，激光显微切割-逆转录酶-聚合酶链反应分析证实了B6所有区域中Ifi 202的高表达。MRLc 1（82-100）肾脏。总之，在Ifi 200家族中，肾脏和免疫器官中的Ifi 202表达随着GN进展而显著增加。Lupus（2010）19，897-905.
B6. MRLc1(82-100) congenic mice carrying the telomeric region of lupus-prone MRL chromosome 1 develop autoimmune glomerulonephritis (GN). The GN susceptibility locus of B6. MRLc1(82-100) contains the interferon activated gene 200 (Ifi200) family, which consists of Ifi202, 203, 204, and 205. Recently, Ifi202 was suggested as a candidate gene for murine lupus. In this study, we assessed the association between Ifi200 family and GN in several disease models. We compared the expression of Ifi200 family members in 24 organs between the C57BL/6 and B6. MRLc1(82-100). The expressions of Ifi200 family members differed between strains, and the most dramatic differences appeared in Ifi202 expression. Briefly, in the blood, immune organs, lungs, and testes mRNA expression was higher in B6. MRLc1(82-100) mice. In the kidney and immune organs, only Ifi202 expression increased with the development of GN in B6. MRLc1(82-100), and significant differences from C57BL/6 were observed even before disease onset. Ifi202 expression in the kidneys of BXSB, NZB/WF1, and MRL/lpr was also significantly high in the early- and late-disease stages. Furthermore, laser microdissection-reverse-transcriptase-polymerase chain reaction analysis confirmed the high Ifi202 expression in all areas of B6. MRLc1(82-100) kidneys. In conclusion, in the Ifi200 family, Ifi202 expressions in the kidney and immune organs significantly increased with GN progression. Lupus (2010) 19, 897-905.