Histologic and rheologic characterization of vocal fold scarring
Histologic and rheologic characterization of vocal fold scarring
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DOI：
10.1016/s0892-1997(02)00078-4
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发表时间：
2002-03-01
期刊：
影响因子：
2.2
通讯作者：
Ford, CN
中科院分区：
文献类型：
--
作者：
Thibeault, SL;Gray, SD;Ford, CN
声带疤痕会导致相当大的发音困难，并提出了重大的治疗挑战。本实验建立了兔声带固有层瘢痕化模型，观察了声带固有层的组织学超微结构和流变学特性。本文对11只兔喉进行了钳夹活检。术后60 d处死动物，取声带组织。完成了瘢痕和正常固有层的胶原蛋白、前胶原蛋白、弹性蛋白和透明质酸的组织学分析。测量了组织的线性粘弹性剪切特性，包括弹性剪切模量和动态粘度。与正常声带固有层相比，瘢痕组织中胶原蛋白明显减少，前胶原蛋白增加，弹性蛋白减少。在流变学上，瘢痕组织的弹性剪切模量和动态粘度均显著较高。硬度和粘度的增加似乎不是胶原蛋白增加的结果，而是似乎与新的、无序的胶原蛋白支架的存在有关。结果被解释的可能作用的间质蛋白质的病因增加的刚度和粘度，这需要进一步调查。这种动物模型应允许系统的声带瘢痕及其治疗的未来调查。
Scarring of the vocal fold causes considerable dysphonia and presents significant treatment challenges. A rabbit model was developed to investigate the histologic ultrastructure and rheologic properties of the scarred vocal fold lamina propria. Eleven rabbit larynges were scarred by means of forcep biopsy. Sixty days postoperatively, the rabbits were sacrificed and their vocal folds were harvested. Histological analysis of the scarred and normal lamina propria was completed for collagen, procollagen, elastin, and hyaluronic acid. Linear viscoelastic shear properties of the tissues were also measured, including elastic shear modulus and dynamic viscosity. Compared to normal vocal fold lamina propria, scarred tissues demonstrated significantly less collagen, an increase in procollagen, and a decrease in elastin. Rheologically, both elastic shear modulus and dynamic viscosity were significantly higher for the scarred tissues. Increased stiffness and viscosity do not appear to result from an increase in collagen, but rather appear to be related to the presence of new, disorganized collagen scaffolding. Results are interpreted in terms of the possible role of interstitial proteins in the etiology of increased stiffness and viscosity, which requires further investigation. This animal model should allow for systematic future investigations of vocal fold scarring and its treatment.