Babesia bigemina sexual stages are induced in vitro and are specifically recognized by antibodies in the midgut of infected Boophilus microplus ticks
Babesia bigemina sexual stages are induced in vitro and are specifically recognized by antibodies in the midgut of infected Boophilus microplus ticks
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DOI：
10.1016/j.ijpara.2004.07.003
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发表时间：
2004-10-01
影响因子：
4
通讯作者：
Figueroa, JV
中科院分区：
文献类型：
--
作者：
Mosqueda, J;Falcon, A;Figueroa, JV
双头巴贝斯虫是一种牛巴贝斯虫病的病原体，只通过受感染的扁虱从一头牛传播到另一头牛。Bigemina的生活史包括硬体寄主的有性阶段；然而，任何巴贝斯虫物种的有性阶段的分子还没有被表征。这是首次报道在体外诱导巴贝斯虫物种的有性阶段，而不含扁虱抗原。用诱导培养液体外培养红细胞内寄生虫20it。红细胞外寄生虫在诱导后3h开始出现；诱导后6h出现长突起的拉长阶段，到9h它们配对并融合形成更大的阶段。诱导后20h出现圆形受精卵。此外，通过使用Percoll梯度，提纯性阶段不受红细胞内阶段的污染。用纯化的寄生虫制备多克隆抗体，该多克隆抗体可与体外诱导的有性期表达的抗原特异性结合，而不与红细胞内表达的抗原特异性结合。重要的是，这些抗体专门识别了喂食受感染牛的微小牛流感雌性动物中肠的性阶段。(C)2004年澳大利亚寄生虫学协会，Elsevier Ltd.出版。版权所有。
Babesia bigemina, a causative agent of bovine babesiosis, is transmitted from one bovine to another only by infected ticks. The life cycle of B. bigemina includes a sexual phase in the tick host; however, molecules from sexual stages of any Babesia species have not been characterized. This is the first report of the induction of sexual stages of any Babesia species in vitro, free of tick antigens. Intraerythrocytic parasites were cultured in vitro for 20 It using an induction medium. Extraerythrocytic parasites were first seen 3 h post induction; elongated stages with long projections appeared at 6 h post induction and by 9 h they paired and fused to form larger stages. Round zygotes appeared 20 h post induction. Moreover, by using Percoll gradients, sexual stages were purified free of contaminating intraerythrocytic stages. Purified parasites were used to generate polyclonal antibodies, which specifically bound to antigens expressed in sexual stages induced in vitro, but not to antigens expressed in intraerythrocytic stages. Importantly, these antibodies specifically identified sexual stages from midguts of female Boophilus microplus ticks fed on infected cattle. (C) 2004 Australian Society for Parasitology Inc. Published by Elsevier Ltd. All rights reserved.