Genes associated with the end of dormancy in grapes
Genes associated with the end of dormancy in grapes
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DOI：
10.1007/s10142-003-0094-6
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发表时间：
2003-11
影响因子：
2.9
通讯作者：
T. Pacey-Miller;K. Scott;E. Ablett;S. Tingey;A. Ching;R. Henry
中科院分区：
文献类型：
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作者：
T. Pacey-Miller;K. Scott;E. Ablett;S. Tingey;A. Ching;R. Henry
构建了葡萄花蕾EST文库，测序了4，270条EST。为了研究基因表达水平随时间的变化，特别是导致芽从休眠中释放的目的，排列文库克隆。将阵列与P33标记的探针杂交，所述探针从每周间隔收集的芽样品产生。这些探针覆盖了从萌芽前9周到芽刚出现后的时间。这些基因的表达模式已被检查。结果发现，数据集中74%的基因与已知蛋白质同源。然后根据它们的主要BLAST匹配将基因分配到功能类别。其中13%与光合作用有关，13%与抗病和防御有关，5%与能量有关，12%与代谢有关，20%与蛋白质生产和加工有关，25%与细胞结构和植物生长有关，其余12%未分类。从先前报告与休眠变化相关的文献中检索到的“候选”基因的选择表达模式进行了评估。经过仔细研究，这些基因中的大多数与细胞内的氧化过程和应激反应有关。这项研究的结果表明，即使在休眠状态下，芽中的基因表达也很高。
A grape bud EST library was constructed and 4,270 ESTs sequenced. The library clones were arrayed for the purpose of investigating the level of gene expression over time, particularly leading up to the buds’ release from dormancy. The arrays were hybridized with P33-labeled probes produced from samples of buds collected at weekly intervals. These probes covered the time from 9 weeks prior to bud burst until just after the emergence of the shoots. Expression patterns from these genes have been examined. It was found that 74% of the genes in the data set were homologous to known proteins. Genes were then assigned to functional categories according to their primary BLAST match. Of these 13% were involved with photosynthesis, 13% with disease resistance and defense, 5% energy, 12% metabolism, 20% protein production and processing, 25% cell structure and plant growth and the remaining 12% were unclassified The expression pattern of a selection of “candidate” genes retrieved from literature previously reporting an association with dormancy changes was assessed. On closer examination most of these genes relate to the oxidative processes and stress responses within the cell. The results of this study show that even in the dormant state, gene expression in the buds is high.